核酸检测与抗体测定对儿童肺炎支原体感染的诊断价值  

Application of Nucleic acid Testing and Colloidal Gold Immunochromatography in the Diagnosis of Mycoplasma Pneumoniae Infection in Children

作　　者：孙光[1] 田甜[1] 吕锦[1] 路秀文[1] Sun Guang;Tian Tian;Lv Jin;Lu Xiuwen(Tianjin Public Security Hospital,Tianjin 300040,China)

机构地区：[1]天津市公安医院,天津300040

出　　处：《医疗装备》2024年第15期24-27,共4页Medical Equipment

摘　　要：目的探究肺炎支原体核酸检测(MP-DNA、MP-RNA)与肺炎支原体抗体测定(MP-IgM)对儿童肺炎支原体感染的诊断价值。方法收集2023年11月至2024年2月医院收治的201例呼吸道疾病患儿的外周血标本和咽拭子,分别采用荧光核酸恒温扩增检测技术检测MP-RNA、取-70℃冻存后咽拭子标本,采用实时荧光定量PCR方法检测MPDNA、采用胶体金免疫层析法检测MP-IgM,将MP-DNA、MP-IgM与金标准(MP-RNA)进行比较,统计检测结果的一致性。结果MP-DNA灵敏度为89.47%,特异度为97.55%,与MPRNA检测结果一致性极好(Kappa=0.87)。MP-IgM灵敏度为34.21%,特异度为93.87%,与MP-RNA检测结果一致性较差(Kappa=0.33)。结论MP-DNA与MP-IgM相比,在肺炎支原体的诊断中具有更高的应用价值,特别是感染初期。对于满足门、急诊对实验室报告及时、高效的需求,可考虑抗体测定与核酸检测法相结合,提高实验室对肺炎支原体的诊断能力。Objective To investigate the diagnostic value of Mycoplasma pneumoniae nucleic acid detection(MP-DNA,MP-RNA)and Mycoplasma pneumoniae antibody measurement(MP-IgM)for Mycoplasma pneumoniae infection in children.Methods Peripheral blood specimens and pharyngeal swabs of 201 children with respiratory diseases admitted to the hospital from November 2023 to February 2024 were collected,and MP-RNA was detected by fluorescent nucleic acid thermostatic amplification detection,pharyngeal swab specimens were taken from-70℃frozen storage,and MP-DNA was detected by real-time fluorescence quantitative PCR,and MP-IgM was detected by colloidal gold immunochromatography,respectively.-DNA and MP-IgM were compared with the gold standard(MP-RNA),and the consistency of the results was statistically determined.Results The sensitivity of MP-DNA was 89.47%,the specificity was 97.55%,and the concordance with MP-RNA results was excellent(Kappa=0.87).The sensitivity of MP-IgM was 34.21%,the specificity was 93.87%,and the concordance with MP-RNA results was poor(Kappa=0.33).Conclusion MP-DNA has higher application value in the diagnosis of Mycoplasma pneumoniae compared with MPIgM,especially in the early stage of infection.For meeting the demand for timely and efficient laboratory reporting in outpatient and emergency clinics,the combination of antibody assay and nucleic acid detection method can be considered to improve the diagnostic ability of the laboratory for Mycoplasma pneumoniae.

关 键 词：肺炎支原体 儿童 核酸检测 免疫球蛋白M 

分 类 号：R446[医药卫生—诊断学]