Understanding and controlling the variables for stromal vascular fraction therapy  

作　　者：Naveen Jeyaraman Sandeep Shrivastava VR Ravi Arulkumar Nallakumarasamy Aditya Pundkar Madhan Jeyaraman 

机构地区：[1]Department of Orthopaedics,Datta Meghe Institute of Higher Education and Research,Wardha 442004,Maharashtra,India [2]Regenerative Medicine,Mother Cell Regenerative Centre,Tiruchirappalli 620017,Tamil Nadu,India [3]Department of Orthopaedics,Jawaharlal Nehru Medical College,Datta Meghe Institute of Higher Education and Research,Wardha 442004,Maharashtra,India [4]Department of Orthopaedics,ACS Medical College and Hospital,Dr MGR Educational and Research Institute,Chennai 600077,Tamil Nadu,India

出　　处：《World Journal of Stem Cells》2024年第8期784-798,共15页世界干细胞杂志（英文版）（电子版）

摘　　要：In regenerative medicine,the isolation of mesenchymal stromal cells(MSCs)from the adipose tissue’s stromal vascular fraction(SVF)is a critical area of study.Our review meticulously examines the isolation process of MSCs,starting with the extraction of adipose tissue.The choice of liposuction technique,anatomical site,and immediate processing are essential to maintain cell functionality.We delve into the intricacies of enzymatic digestion,emphasizing the fine-tuning of enzyme concentrations to maximize cell yield while preventing harm.The review then outlines the filtration and centrifugation techniques necessary for isolating a purified SVF,alongside cell viability assessments like flow cytometry,which are vital for confirming the efficacy of the isolated MSCs.We discuss the advantages and drawbacks of using autologous vs allogeneic SVF sources,touching upon immunocompatibility and logistical considerations,as well as the variability inherent in donor-derived cells.Anesthesia choices,the selection between hypo-dermic needles vs liposuction cannulas,and the role of adipose tissue lysers in achieving cellular dissociation are evaluated for their impact on SVF isolation.Centrifugation protocols are also analyzed for their part in ensuring the integrity of the SVF.The necessity for standardized MSC isolation protocols is highlighted,promoting reproducibility and successful clinical application.We encourage ongoing research to deepen the understanding of MSC biology and therapeutic action,aiming to further the field of regenerative medicine.The review concludes with a call for rigorous research,interdisciplinary collaboration,and strict adherence to ethical and regulatory standards to safeguard patient safety and optimize treatment outcomes with MSCs.

关 键 词：Mesenchymal stromal cells Stromal vascular fraction Adipose tissue Autologous stromal vascular fraction Stromal vascular fraction isolation 

分 类 号：R684.3[医药卫生—骨科学]