生理盐水洗涤DIC患者凝集红细胞用于血型鉴定的效果评价  

Effect evaluation of using normal saline to wash red blood cells for blood type identification in patients with disseminated intravascular coagulation

作　　者：杨永刚[1] 范秀清 陈松[1] YANG Yonggang;FAN Xiuqing;CHEN Song(Clinical Laboratory Department,Hangzhou Third People's Hospital in Zhejiang Province,Hangzhou 310009,China;Clinical Laboratory Department,Zhejiang Medical Health Group Hangzhou Hospital(Hanggang Hospital),Hangzhou 310022,China)

机构地区：[1]浙江省杭州市第三人民医院检验科,杭州310009 [2]浙江省医疗健康集团杭州医院(杭钢医院)检验科,杭州310022

出　　处：《浙江医学教育》2024年第4期246-250,共5页Zhejiang Medical Education

摘　　要：目的探讨弥散性血管内凝血(disseminated intravascular coagulation,DIC)患者凝集红细胞生理盐水洗涤后血型鉴定的效果。方法选择浙江省杭州市第三人民医院2019年7月—2022年7月由于自身红细胞凝集导致血型鉴定困难的24例DIC患者为研究对象,ABO血型以基因分型结果为标准,生理盐水洗涤凝集红细胞前后手工方法(试管法和微胶柱法)ABO血型鉴定结果与标准组比较。结果凝集红细胞洗涤前ABO血型2例正反定型符合,与标准结果比较,其差异具有统计学意义(P<0.05)。生理盐水洗涤后的红细胞均匀散开,24例ABO血型检测结果正反定型符合,其中A型8例,B型5例,O型9例,AB型2例。洗涤后血型结果与标准结果比较,其差异无统计学意义(P>0.05)。结论采用生理盐水洗涤凝集红细胞的方法,血型鉴定效果良好,具有明显检测优势,为临床紧急用血提供了安全保障。Objective To evaluate the effectiveness of blood type identification after red blood cell washing in patients diagnosed with disseminated intravascular coagulation(DIC)caused by self-aggregating red blood cells.Methods A total of 24 samples from DIC patients with self-aggregation red blood cell from July 2019 to July 2022 in Hangzhou Third People's Hospital in Zhejiang Province were collected.ABO blood type was based on genotyping results.The results of ABO blood type identification by tube method and micro-column gel agglutination assay(MGAA)before and after washing aggregated red blood cells with normal saline were compared with those of the standard group.Results The positive and negative ABO blood types of 2 cases were coincident before red blood cell washing,and there was significant difference between the results and the standard results(P<0.05).The red blood cells dispersed evenly after washing with normal saline.The results of ABO blood type were positive and negative in 24 cases,including 8 cases of type A,5 cases of type B,9 cases of type O and 2 cases of type AB.There was no significant difference between the standard results and the blood type results after washing(P>0.05).Conclusions The method of washing red blood cells with normal saline has a good blood type identification effect,which has obvious advantages in detection,and provides a safety guarantee for clinical emergency blood use.

关 键 词：自身红细胞凝集 红细胞洗涤 血型 弥散性血管内凝血 

分 类 号：R457.11[医药卫生—治疗学]