Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis  

作　　者：Li Liang Fuming Zhang Quanhong Li Baoguo Sun Yuyu Zhang Robert J.Linhardt 

机构地区：[1]Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Key Laboratory of Flavor Chemistry,Beijing Technology and Business University(BTBU),Beijing 100048,China [2]Departments of Chemical and Biological Engineering,Chemistry and Chemical Biology,Biomedical Engineering and Biological Science,Center for Biotechnology and Interdisciplinary Studies,Rensselaer Polytechnic Institute,Troy 12180,USA [3]College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China [4]China National Engineering Research Center for Fruit and Vegetable Processing,Beijing 100083,China

出　　处：《Food Science and Human Wellness》2024年第5期2937-2946,共10页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(32122069 and 31972191);the Beijing Outstanding Young Scientist Program(BJJWZYJH01201910011025);funded by National Institutes of Health Grants DK111958 and AG062344 to R.J.L.

摘　　要：The present study reports the structural characteristics of 3 polysaccharide fractions(SPS-F1,SPS-F2 and SPS-F3)isolated and purified from squash.SPS-F1(molecular weight(Mw)=12.30 kDa)and SPS-F2(Mw=19.40 kDa)were likely to contain HG and RG-I domain of pectic polysaccharide,respectively.SPS-F2(Mw=270.4 kDa)was mainly composed of rhamnose,galactose and arabinose.The treatment with SPS decreased body weight gain,glucose and TG levels in type 2 diabetes rats.Besides,25 differential metabolites were identified based on urinary metabolomics analysis,which are crucial to the anti-diabetic effect of SPS.The regulation of nicotinamide N-oxide,histamine,cis-aconitate,citrate,L-malic acid,3-(3-hydroxyphenyl)propanoic acid and N-acetyl-L-aspartic acid were mainly associated with energy metabolism,gut microbiota and inflammation.Study of surface plasmon resonance revealed the binding kinetics with galectin-3(Gal-3)and fibroblast growth factor 2(FGF2).The K_(D)values of SPS-F2 and SPS-F3 to Gal-3 were 4.97×10^(-3)and 1.48×10^(-3)mol/L,indicating a weak binding affinity.All 3 fractions showed moderate binding to FGF2 and the affinity was SPS-F3>SPS-F2>SPS-F1.Thus,the metabolomics and SPR approach were proved to be a promising tool in exploring the anti-diabetes effects of SPS and provided a deep understanding of the mechanisms.

关 键 词：POLYSACCHARIDE Metabolomics Type 2 diabetes Surface plasmon resonance 

分 类 号：R587.1[医药卫生—内分泌] TS201.4[医药卫生—内科学]