2株耐盐碱根际促生细菌对盐碱胁迫下紫花苜蓿生长及生理的影响  

Effects of two saline-tolerant rhizosphere growthpromoting bacteria on growth and physiology of Medicago sativa under saline-alkali stress

作　　者：陈露 马南楠 陈宁 曲善民[1,2,3] 苗阳阳 CHEN Lu;MA Nan-nan;CHEN Ning;QU Shan-min;MIAO Yang-yang(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Heilongjiang Province Cultivating Collaborative Innovation Center for The Beidahuang Modern Agricultural Industry Technology,Daqing 163319,China;Key Laboratory of Efficient Utilization of Feed Resources and Nutrition manipulation in Cold Region of Heilongjiang Province,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]北大荒现代农业产业技术协同创新中心,黑龙江大庆163319 [3]黑龙江省寒区饲料资源高效利用与营养调控重点实验室,黑龙江大庆163319

出　　处：《草原与草坪》2024年第4期197-203,共7页Grassland and Turf

基　　金：黑龙江八一农垦大学学成、引进人才科研启动计划项目(XYB201813);黑龙江八一农垦大学三横三纵支持计划项目(ZRCPY201908);黑龙江八一农垦大学教学研究课题(NDJY2141);大庆市新能源领域“揭榜挂帅”科技攻关项目(2021BD05);黑龙江省科技厅省院合作项目(YS16B12)。

摘　　要：【目的】研究接种耐盐碱根际促生细菌嗜麦芽寡养单胞菌JT4、普城沙雷氏菌JG1对盐碱胁迫条件下紫花苜蓿生长和生理特性的影响,为根际促生细菌JT4、JG1在植物抗盐碱中的应用提供理论依据。【方法】采用室内盆栽试验,以紫花苜蓿为供试植物,以实验室前期筛选鉴定得到的耐盐碱及促生能力均较好的根际促生细菌JT4、JG1为供试菌株,设置盐碱胁迫及非盐碱胁迫处理,测定接种根际促生细菌JT4、JG1后紫花苜蓿幼苗生长和生理特性指标。【结果】与对照相比,盐碱胁迫下接种JT4、JG1紫花苜蓿幼苗的株高分别提高了31.64%和43.50%,根长分别提高了32.78%和7.05%,鲜重分别提高了100%和60%,抗坏血酸过氧化物酶活性分别提高了27.67%和200.58%,过氧化氢酶活性分别提高了33.33%和22.73%,可溶性蛋白含量分别提高了126.94%和118.88%,叶绿素a含量分别提高了11.12%和16.32%。【结论】耐盐碱根际促生细菌JT4、JG1能较好地缓解盐碱胁迫对紫花苜蓿造成的损伤。【Objective】In this experiment,the effects of inoculation of halotolerant rhizosphere growth-promoting bacteria,Stenotrophomonas maltophilia JT4 and Serratia plymuthica JG1,on plant growth and physiological charac-teristics of Medicago sativa under saline-alkali stress were studied,so as to provide theoretical basis for the applica-tion of rhizosphere growth-promoting bacteria JT4 and JG1 in saline-alkali resistance of plants.【Method】In indoor pot experiment,alfalfa was used as the test plant,and rhizosphere growth-promoting bacteria JT4 and JG1 with good saline-alkali tolerance and growth-promoting ability were used as the test strains.The effects of inoculation of rhizo-sphere growth-promoting bacteria JT4 and JG1 on the growth and physiological characteristics of alfalfa seedlings were studied under saline-alkali stress and non-saline stress.【Result】Compared with the control,the plant height,root length,fresh weight,ascorbic acid peroxidase activity and catalase activity of alfalfa seedlings inoculated with JT4 and JG1 increased by 31.64%and 43.50%,32.78%and 7.05%,100%and 60%,27.67%and 200.58%,and 33.33%and 22.73%respectively,soluble protein content increased by 126.94%and 118.88%,and chlorophyll a content increased by 11.12%and 16.32%,respectively.【Conclusion】Saline-alkali tolerant rhizosphere growth-pro-moting bacteria JT4 and JG1 can better alleviate the damage of alfalfa caused by saline-alkali stress.

关 键 词：根际促生细菌 紫花苜蓿 盐碱胁迫 抗氧化活性 

分 类 号：S541[农业科学—作物学]