新疆喀什地区腹泻羔羊源产ESBL大肠杆菌的耐药性和系统进化分群研究  

Drug resistance and phylo-typing of ESBL-producing Escherichia coli from diarrheic lambs in Kashgar area,Xinjiang

作　　者：胡芸 郑百利 陈伟丽 程雅玲 马兰 佟盼盼 刘英玉[1,2] HU Yun;ZHENG Bai-li;CHEN Wei-li;CHENG Ya-ling;MA Lan;TONG Pan-pan;LIU Ying-yu(College of Veterinary Medicine,Xinjiang Agricultural University,Urumuqi 830000,China;Xinjiang Key Laboratory of Herbivore Drug Research and Creation,Urumuqi 830000,China;Urumqi County Agricultural Products Quality and Safety Testing Center,Urumuqi 830000,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830000 [2]新疆草食动物新药研究与创制重点实验室,乌鲁木齐830000 [3]乌鲁木齐县农产品质量安全检测中心,乌鲁木齐830000

出　　处：《中国人兽共患病学报》2024年第8期716-722,共7页Chinese Journal of Zoonoses

基　　金：新疆肉牛肉羊高效生产及产品加工技术集成创新示范项目(No.2021A02003);自治区“三农”骨干人才培养项目(No.2022SNGGGCC015)联合资助。

摘　　要：目的了解新疆喀什地区腹泻羔羊源产ESBL大肠杆菌的流行情况及耐药性,指导临床大肠杆菌病的防治。方法采集385份喀什地区腹泻羔羊肛周粪便,分离得到371株大肠杆菌,通过双纸片协同法筛选产ESBL大肠杆菌,对筛选到的菌株进行耐药基因鉴定、耐药性分析和系统进化分群研究。结果371株大肠杆菌中204株为产ESBL菌株,bla CTX-M、bla CTX-M-1G、bla CTX-M-9G和bla TEM耐药基因携带率分别为67.65%、69.12%、30.39%和63.73%,产ESBL阳性菌株均为多重耐药,对氨苄西林、头孢噻肟、庆大霉素、恩诺沙星、阿奇霉素、四环素、氯霉素、甲氧苄啶、氨曲南8种药物的耐药率为90.69%~100%,系统进化分群主要分布于A群和D群,其中A群菌株以10重耐药为主(41.11%),D群菌株以11重耐药为主(40%)。结论产ESBL菌株大肠杆菌是引起喀什地区羔羊腹泻的主要病原菌,以A群为主,且均为多重耐药。The objective of this study was to determine the frequency and resistance patterns of ESBL-producing E.coli in lambs with diarrhea in the Kashi area,Xinjiang.The findings may provide guidance for the prevention and control of clinical E.coli disease.We collected 385 samples of perianal feces from lambs with diarrhea in the Kashgar area.From these samples,we isolated 371 strains of E.coli.We then used the double-paper-sheet synergistic method to screen for ESBL-producing E.coli.Additionally,we conducted analyses to identify drug-resistance genes,analyze drug resistance,and study the phylo-typing of the screened strains.Of 371 E.coli strains,204 were identified as ESBL-producing strains.The prevalence rates of bla CTX-M,bla CTX-M-1G,bla CTX-M-9G,and bla TEM resistance genes was 67.65%,69.12%,30.39%,and 63.73%,respectively.All ESBL-producing strains were resistant to multiple drugs,with resistance rates ranging from 90.69%to 100%for eight specific drugs:ampicillin,cefotaxime,gentamicin,enrofloxacin,azithromy-cin,tetracycline,chloramphenicol,methotrexate,and amitrazine.The phylogenetic subgroups of the strains were distributed primarily in groups A and D.Among group A strains,41.11%exhibited resistance to ten drugs,whereas among group D strains,40%exhibited resistance to 11 drugs.ESBL-producing strains of Escherichia coli are the main pathogens causing diarrhea in lambs in the Kashgar region;group A is the main group,and all groups are multi-drug resistant.

关 键 词：腹泻羔羊 大肠杆菌 ESBL 药敏试验 系统进化分群 

分 类 号：R378.2[医药卫生—病原生物学]