2014-2018年江苏省人源喹诺酮耐药1,4,[5],12:i:-沙门氏菌的基因组学初步分析  

Genomic analysis of human quinolone resistant 1,4,[5],12:i:-Salmonella in Jiangsu Province from 2014 to 2018

作　　者：郑东宇[1,2,3] 马恺 周翌婧[1] 吴高林 霍翔[1,2,3] 乔昕[1,3] ZHENG Dong-yu;MA Kai;ZHOU Yi-jing;WU Gao-lin;HUO Xiang;QIAO Xin(Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China;NHC Key Laboratory of Enteric Pathogenic Microbiology,Nanjing 210009,China;Jiangsu Key Laboratory of Pathogenic Microorganisms of Emerging Major Infectious Diseases,Nanjing 210009,China)

机构地区：[1]江苏省疾病预防控制中心,南京210009 [2]国家卫生健康委员会肠道病原微生物重点实验室,南京210009 [3]江苏省新发突发重大传染病病原微生物重点实验室,南京210009

出　　处：《中国人兽共患病学报》2024年第8期739-744,共6页Chinese Journal of Zoonoses

基　　金：江苏省卫健委科研项目(No.M2021078)。

摘　　要：目的分析江苏省喹诺酮耐药1,4,[5],12:i:-沙门氏菌分子流行病学特征。方法用cgMLST分析江苏省及欧美来源菌株的分子流行病学特征,并初步分析喹诺酮耐药1,4,[5],12:i:-沙门氏菌可能的传播特征。结果13株喹诺酮耐药1,4,[5],12:i:-沙门氏菌共注释11大类抗性基因(Antibiotic resistance genes,ARGs)。其中氨基糖苷类ARGs检出率最高(100%);12株喹诺酮耐药菌株(92.3%)均携带IncHI2/IncHI2A质粒类型;不同国家/地区来源菌株质粒介导喹诺酮耐药(PMQR)基因携带分析显示美国及欧洲来源菌株携带6类PMQR基因,qnrB19检出率最高。江苏省来源菌株携带3类PMQR基因类型,aac(6′)-Ib-cr检出率最高(11.84%);不同国家/地区来源菌株cgMLST位点差异分析显示形成3个主要流行谱系。结论江苏省人源喹诺酮耐药1,4,[5],12:i:-沙门氏菌分离株与欧美菌株可能具有共同进化来源,PMQR基因携带水平存在国家/地区差异。The molecular epidemiological characteristics of 1,4,[5],12:i:-Salmonella in Jiangsu Province were analyzed through whole genome sequencing(WGS).The distribution characteristics of related genes were obtained on the basis of annotated drug-resistant genes and plasmid types in the whole genome.Analysis of the molecular epidemiological characteristics of strains with cgMLST revealed possible modes of transmission of quinolone resistance in 1,4,[5],12:i:-Salmonella.Eleven categories of antibiotic resistance genes(ARGs)were annotated among the fluoroquinolone-resistant strains.The detection rate of aminoglycoside ARGs was highest(100%).Twelve quinolone-resistant strains(92.3%)carried the IncHI2/IncHI2A plasmid type.PMQR gene analysis of various strains indicated that the strains from the United States and Europe carried six types of PMQR genes,and the detection rate of qnrB19 was highest.The Jiangsu strains carried three PMQR gene types,and the detection rate of aac(6′)-Ib-cr was highest(11.84%).Analysis of cgMLST loci from different countries/regions revealed three main epidemic clusters.Some isolates from Jiangsu province might have the same evolutionary origin as some isolates from Europe and the United States,and national/regional differences were observed in the PMQR gene carriage level.

关 键 词：1 4 [5] 12:i:-沙门氏菌 喹诺酮耐药 分子流行病学 

分 类 号：R378.2[医药卫生—病原生物学]