磁场对血红蛋白氧合速率的影响  

The Magnetic Field Effects on The Oxygenation Rate of Recombinant Hemoglobin

作　　者：刘颜 方彦雯 张欣[2] 谢灿 LIU Yan;FANG Yan-Wen;ZHANG Xin;XIE Can(College of Life Sciences,Anhui Medical University,Hefei 230032,China;High Magnetic Field Laboratory,Hefei Institute of Physical Sciences,Chinese Academy of Sciences,Hefei 230031,China;Heye Health Industrial Research Institute of Heye Health Technology Co.,Ltd.,Huzhou 313300,China)

机构地区：[1]安徽医科大学生命科学学院,合肥230032 [2]中国科学院合肥物质科学研究院强磁场科学中心,合肥230031 [3]和也健康科技有限公司和也智慧健康研究院,湖州313300

出　　处：《生物化学与生物物理进展》2024年第9期2210-2221,共12页Progress In Biochemistry and Biophysics

基　　金：中国科学院合肥物质科学研究院院长基金(Y96XC11131)资助项目。

摘　　要：目的血红蛋白是高等动物体内运输氧气的载体蛋白,本工作拟探究磁场对血红蛋白氧合的影响,并比较人和牦牛血红蛋白在磁场下氧合的差异。方法构建人和牦牛血红蛋白的重组表达纯化系统;分别将人和牦牛脱氧血红蛋白置于地磁场或外加磁场下,通过收集紫外可见光谱数据,计算氧合血红蛋白、脱氧血红蛋白和高铁血红蛋白3种组分的浓度和比例,分析磁场对血红蛋白氧合速率的影响;使用超导量子干涉磁测量系统SQUID对人氧合血红蛋白和人脱氧血红蛋白进行磁性测量,探讨磁场影响血红蛋白氧合速率的可能机制。结果利用大肠杆菌原核表达系统成功表达并纯化得到了人和牦牛的血红蛋白样品。发现牦牛血红蛋白比人血红蛋白的氧合速率更快,外加0.3 T静磁场显著提高了血红蛋白的氧合速率,且牦牛血红蛋白比人血红蛋白对磁场更为敏感。结论本研究成功表达和纯化了人和牦牛血红蛋白,发现磁场促进了血红蛋白的氧合过程。磁场对血红蛋白氧合速率的影响可能与血红蛋白本身的磁学性质有关。这些发现为未来使用外加磁场作为辅助手段改善低氧症状提供了理论支撑,有望在临床中发挥作用。Objective Hemoglobin is the iron-containing protein in the red blood cells of many animals.The primary function of hemoglobin is to transport oxygen from lung to tissues.It is composed of two identicalα-globin subunits and two identicalβ-globin subunits.Hemoglobin has unique magnetic properties.The paramagnetism of deoxyhemoglobin,and the diamagnetism of oxyhemoglobin and carboxyhaemoglobin have been reported previously.Studies have also shown that external magnetic field affected blood flow rate,but whether magnetic field may affect the oxygenation rate of hemoglobin remains unknown.Here in this study,we are aiming to address this question with recombinant hemoglobin.Human hemoglobin and yak hemoglobin were selected as the research objects,and a recombinant protein expression and purification system was established to explore the magnetic field effects on the oxygenation rate of hemoglobin,as well as the differences in the oxygenation rate between human hemoglobin and yak hemoglobin under external magnetic field.Methods The recombinant expression and purification system of human and yak hemoglobin was established.The recombinant hemoglobin expression was further optimized and appropriated inducing temperature and IPTG concentration were screened.Recombinant human hemoglobin and yak hemoglobin were purified to homogeneity by affinity chromatography and further by size-exclusion chromatography.SDS-PAGE was used to validate the purification,and UV-Vis spectrum and EPR were used to characterize the biochemical properties of recombinant hemoglobin.Deoxyhemoglobin of human and yak were placed under 0.3 T external magnetic field to test the magnetic field effects on oxygenation rate,and geomagnetic field condition was used as a sham control.The UV-Vis spectrum data were measured every 10 min,and the concentration and proportion of oxygenated hemoglobin,deoxyhemoglobin and methemoglobin were calculated to analyze the effects of magnetic field on the oxygenation rate of hemoglobin.The magnetic properties of human oxyge

关 键 词：血红蛋白 磁场 人血红蛋白 牦牛血红蛋白 氧合速率 

分 类 号：Q7[生物学—分子生物学]