间断theta脉冲磁刺激对空间工作记忆相关神经振荡及其同步性的影响  

Effect of Intermittent Theta Burst Stimulation on Spatial Working Memory-related Neural Oscillations and Their Synchronicity

作　　者：郭苗苗[1,2,3,4] 张彪[1,2,3,4] 张天恒 王田 翟昊迪 徐桂芝[1,2,3,4] GUO Miao-Miao;ZHANG Biao;ZHANG Tian-Heng;WANG Tian;ZHAI Hao-Di;XU Gui-Zhi(School of Health Science and Biomedical Engineering,Hebei University of Technology,Tianjin 300130,China;State Key Laboratory of Reliability and Intelligence of Electrical Equipment,Hebei University of Technology,Tianjin 300130,China;Tianjin Key Laboratory of Bioelectromagnetic Technology and Intelligent Health,Hebei University of Technology,Tianjin 300130,China;Hebei Key Laboratory of Bioelectromagnetics and Neuroengineering,Hebei University of Technology,Tianjin 300130,China;School of Mechanical and Electrical Engineering,Shijiazhuang University,Shijiazhuang 050035,China)

机构地区：[1]河北工业大学生命科学与健康工程学院,天津300130 [2]河北工业大学,省部共建电工装备可靠性与智能化国家重点实验室,天津300130 [3]河北工业大学,天津市生物电工与智能健康重点实验室,天津300130 [4]河北工业大学,河北省生物电磁学与神经工程重点实验室,天津300130 [5]石家庄学院机电学院,石家庄050035

出　　处：《生物化学与生物物理进展》2024年第9期2222-2239,共18页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(52320105008);国家重点研发计划(2022YFC2402203);河北省自然科学基金(E2021202222,F2024202085);河北省科技计划(18963001D,215676146H,225676163GH);天津市自然科学基金(20JCQNJC00710,21JCXJC00090);河北工业大学电气设备可靠性与智能化国家重点实验室(EERI_OY2021009)资助项目。

摘　　要：目的间断theta脉冲磁刺激(iTBS)已被证实可以通过夹带大脑不同频段神经振荡调控工作记忆功能,但其对神经振荡及其同步性的调节尚不清晰。本研究旨在探讨iTBS对大鼠局部脑区及跨脑区神经振荡及其同步性的影响,探究iTBS调控工作记忆的作用机制。方法24只大鼠根据年龄及是否接受iTBS分为4组,应用时频分布、相位同步性以及相位-幅值耦合分析方法,对比分析各组大鼠在进行空间工作记忆(SWM)行为学任务的过程中前额叶和海马脑区theta及gamma频段局部场电位信号神经振荡的变化规律。结果随着年龄增长,老年大鼠学会SWM任务规则所用时间明显增加(P=0.0056);iTBS可以缩短成年大鼠(P=0.0011)和老年大鼠(P=0.0090)学会SWM任务规则所用时间。相比成年大鼠,老年大鼠前额叶和海马脑区theta和gamma频段神经振荡的时频能量(theta:P<0.0001;gamma:P<0.0001)、跨脑区相位-幅值耦合作用(PFC-HPC:P=0.0002;HPC-PFC:P=0.0277)均出现一定程度的下降;iTBS可以提高成年大鼠(theta:P<0.0001;gamma:P<0.0001)和老年大鼠(theta:P=0.0144;gamma:P=0.0006)神经振荡的时频能量以及老年大鼠跨脑区相位-幅值耦合作用(PFC-HPC:P=0.0180;HPC-PFC:P=0.0221)。此外,两脑区各频段信号的时频能量及相位-幅值耦合与大鼠行为学正确率均具有正相关关系。结论iTBS可以增强老年大鼠的SWM能力以及认知功能,这与老年大鼠SWM任务期间跨脑区theta频段和gamma频段神经振荡的时频能量以及跨脑区相位幅值耦合增强有关。对于成年大鼠,iTBS通过提升成年大鼠SWM任务期间两脑区theta频段和gamma频段神经振荡的时频能量增强其SWM能力与认知功能。Objective Spatial working memory(SWM)is an important function in cognitive behavior,and working memory impairment can seriously affect the patient’s life and cause great stress to the patient.Intermittent theta burst stimulation(iTBS)has been shown to regulate working memory function by entrainment of neural oscillations in different frequencies of the brain,but its regulation of working memory-related neural oscillations and their synchronization is not clear.The purpose of this study was to study the effect of iTBS on neural oscillation and synchronization in local and transbrain regions of rats,and to explore the mechanism of iTBS in regulating working memory.Methods Twenty-four rats were randomly divided into four groups according to their age and whether they received iTBS stimulation(AS:adult stimulation group,AC:adult control group,ES:elderly stimulation group,EC:elderly control group).Using the methods of time-frequency distribution,phase synchronization and phase-amplitude coupling analysis,the changes of local field potential signal neural oscillations in the prefrontal and hippocampal brain regions of theta and gamma bands in the process of spatial working memory behavioral tasks in each group of rats were compared and analyzed,and the relationship between the changes of neural oscillations in the two brain regions and the changes in spatial working memory ability of rats was judged based on the Pearson correlation coefficient.Results With the increase of age,the time taken by the elderly rats to learn the spatial working memory task rules increased significantly(P=0.0056),and the time taken by iTBS stimulation to learn the SWM task rules in adult rats(P=0.0011)and elderly rats(P=0.0090)was shortened.At the same time,compared with adult rats,the time-frequency energy of theta and gamma band neural oscillations in the prefrontal and hippocampal brain regions of elderly rats(theta:P<0.0001;gamma:P<0.0001)and phase-amplitude coupling across brain regions(PFC-HPC:P=0.0002;HPC-PFC:P=0.0277)decreased to a

关 键 词：间断theta脉冲磁刺激 空间工作记忆 神经振荡 相位同步性 相位-幅值耦合 

分 类 号：R318[医药卫生—生物医学工程] R338[医药卫生—基础医学]