糙皮侧耳BAG家族蛋白鉴定及其基因表达模式分析  

Identification and Expression Pattern Analysis of BAG Family in Pleurotus ostreatus

作　　者：于振 田甜 刘元栋[1,2] 麦非凡 胡延如 文晴 戚元成[1] 王风芹[1] YU Zhen;TIAN Tian;LIU Yuandong;MAI Feifan;HU Yanru;WEN Qing;QI Yuancheng;WANG Fengqin(College of Life Sciences,Henan Agricultural University,Zhengzhou 450046,Henan,China;Academy of Agricultural Sciences of Zhoukou,Zhoukou 466000,Henan,China)

机构地区：[1]河南农业大学生命科学学院,河南郑州450046 [2]周口市农业科学院,河南周口466000

出　　处：《食用菌学报》2024年第5期17-26,共10页Acta Edulis Fungi

基　　金：国家现代农业产业技术体系(CARS-20);河南省农业良种联合攻关项目(2022030101)。

摘　　要：在NCBI、JGI数据库检索糙皮侧耳(Pleurotus ostreatus)BAG蛋白(PoBAGs),对其进行生物信息学分析,并测定不同发育(菌丝、原基、子实体)阶段和高温(42℃)、低温(4℃)胁迫时PoBAG基因的表达情况,分析其表达模式。结果表明:共检索到3个(分别命名为PoBAG-1、PoBAG-2、PoBAG-3)具有BAG结构域的蛋白,其中,PoBAG-1、PoBAG-2还分别含有1个IQ基序和类泛素化结构域;PoBAG-1、PoBAG-3为亲水性不稳定蛋白,且亲缘关系较近,可能主要定位于细胞核和细胞质中;PoBAG-2为亲水性稳定蛋白,与其他具有类泛素化结构域的BAG蛋白具有较近的亲缘关系,可能主要定位于线粒体和细胞质中。与菌丝相比,3个PoBAGs在原基和子实体中相对表达量均显著上调;与对照(25℃)相比,42℃高温胁迫时,PoBAG-1、PoBAG-3相对表达量极显著上调,PoBAG-2显著下调;4℃低温胁迫时,PoBAG-1和PoBAG-2相对表达量显著上调,PoBAG-3显著下调。研究结果为进一步解析BAG家族蛋白在糙皮侧耳生长发育以及温度胁迫响应过程中的功能提供参考。The BAG domain-containing proteins(BAGs)of Pleurotus ostreatus(PoBAGs)were retrieved from NCBI and JGI databases,analyzed by bioinformatics tools,and then measured for expression at different developmental stages(mycelium,primordium,fruiting body)and under temperature stresses(high temperature at 42℃and low temperature at 4℃).The results showed that a total of three proteins,named PoBAG-1,PoBAG-2 and PoBAG-3,contain the BAG domain.In addition,PoBAG-1 and PoBAG-2 also contain an IQ motif and a ubiquitin-like(UBL)domain,respectively.PoBAG-1 and PoBAG-3 are hydrophilic unstableand phylogenetically close.They were predicted to be mainly localized in the nucleus and cytoplasm.PoBAG-2is a hydrophilic stable protein closely related to BAG proteins with UBL domain in other organisms.It was predicted to be mainly localized in mitochondria and cytoplasm.The relative expression of the three PoBAGs were significantly up-regulated in primordium and fruiting body compared with mycelium.Compared with the control(25℃),high temperature(42℃)stress resulted in extremely significantly increased relative expression levels of PoBAG-1 and PoBAG-3,and significantly decreased PoBAG-2 expression.Under low temperature(4℃)stress,the relative expression levels of PoBAG-1 and PoBAG-2 were significantly increased while PoBAG-3 was significantly down-regulated.This study provided a reference for further analysis of the function of PoBAG family proteins in the growth and development of P.ostreatus,and its response to temperature stress.

关 键 词：糙皮侧耳 BAG家族蛋白 QRT-PCR 生长发育 温度胁迫 

分 类 号：S646[农业科学—蔬菜学]