基于灰树花基因组的SSR分子标记开发  

Development of SSR Molecular Markers Based on Grifola frondosa Genome

作　　者：高章会 华蓉[1,2] 孙达锋 李雪松[2] 岳万松 刘绍雄 GAO Zhanghui;HUA Rong;SUN Dafeng;LI Xuesong;YUE Wansong;LIU Shaoxiong(Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperatives,Kunming 650221,Yunnan,China;Yunnan Institute of Edible Fungi Industry Development,Kunming 650021,Yunnan,China)

机构地区：[1]中华全国供销合作总社昆明食用菌研究所,云南昆明650221 [2]云南省食用菌产业发展研究院,云南昆明650221

出　　处：《食用菌学报》2024年第5期27-36,共10页Acta Edulis Fungi

基　　金：云南省省市一体化专项(202402AN360003);云南省科技人才与平台计划(202305AD160051);云南省创新引导与科技型企业培育计划(202204BP090012);国家重点研发计划课题(2022YFD1200602)。

摘　　要：以12个灰树花(Grifola frondosa)菌株及已公布的灰树花基因组为基础,采用生物信息学和实验验证相结合的方法,设计192对SSR引物研究灰树花基因组SSR类型及分布。结果表明:总计检索到5种SSR类型的666个位点,其中,三核苷酸重复的SSR位点最多(52.70%),是灰树花SSR的主要类型;二核苷酸SSR位点占比为32.43%;四、五、六核苷酸重复类型较多,但数量较少。由验证实验得到15对SSR引物,共检测到55个等位基因位点,15对SSR引物的平均Shannon’s信息指数、观测杂合度、期望杂合度和多态性信息含量分别为0.817、0.506、0.442和0.397。基于15对SSR引物进行灰树花DNA指纹编码构建,获得12个灰树花菌株唯一的30位数分子指纹数据库编码,可对每一个灰树花菌株进行精准鉴定。Based on 12 Grifola frondosa strains and the published genomic sequence of G.frondosa,192 pairs of SSR primers were designed to study the types and distribution of SSR markers in the G.frondosa,genome and the SSR primers were validated by PCR.The results showed that a total of 666 SSR loci of five SSR types were retrieved.Among them,trinucleotide repeats are the most abundant in G.frondosa,accounting for 52.70%,and the proportion of dinucleotide SSR loci is 32.43%.Repeats of four,five and six bases have a high diversity of repeat motifs,but their quantity was low.Fifteen pairs of polymorphic primers were selected from the validation test,and a total of 55 allele loci were detected.The average Shannon's information index,observed heterozygosity,expected heterozygosity and polymorphism information content of the 15 pairs of SSR primers were 0.817,0.506,0.442 and 0.397,respectively.Using the 15 pairs of SSR primers,the DNA fingerprint coding of G.frondosa was constructed,and the unique 30-digit molecular fingerprint database coding for the 12 G.frondosa strains was obtained,facilitating accurate identification of G.frondosa strains.

关 键 词：灰树花 基因组 SSR 标记开发 

分 类 号：S646[农业科学—蔬菜学]