普通烟草GRAS基因家族鉴定及表达模式分析  

Genome-Wide Identification and Expression Analysis of GRAS Gene Family in Nicotiana tabacum L.

作　　者：孙晋浩 吴昌健 管恩森[3] 熊党安 周天宇 陈悦[1] 刘晓姗 赵清海[3] 王大海[3] 李志远 马志远[1] 田震 SUN Jinhao;WU Changjian;GUAN Ensen;XIONG Dang’an;ZHOU Tianyu;CHEN Yue;LIU Xiaoshan;ZHAO Qinghai;WANG Dahai;LI Zhiyuan;MA Zhiyuan;TIAN Zhen(Technology Center,China Tobacco Jiangsu Industrial Co.,Ltd.,Nanjing 210019,China;Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Shandong Weifang Tobacco Co.,Ltd.,Weifang 261205,Shandong,China)

机构地区：[1]江苏中烟工业有限责任公司技术中心,南京210019 [2]中国农业科学院烟草研究所,青岛266101 [3]山东潍坊烟草有限公司,山东潍坊261205

出　　处：《中国烟草科学》2024年第4期7-18,共12页Chinese Tobacco Science

基　　金：中国烟草总公司重大科技项目(110202201014);中国烟草总公司“揭榜挂帅”项目(110202103015);中国烟草总公司山东省公司科技项目(201909);江苏中烟科技项目(H202110)。

摘　　要：GRAS转录因子是植物中特有的转录因子,在植物的生长发育、信号转导、生物和非生物胁迫响应中均起着重要作用。本研究利用生物信息学手段从普通烟草基因组中鉴定得到了95个烟草GRAS基因,其中有50个GRAS家族成员不均匀地分布在20条染色体上。从系统进化、共线性和表达模式等方面对烟草GRAS基因家族成员进行了深入分析。结果表明,烟草GRAS基因可划分为8个亚家族,共有26个烟草GRAS成员源自全基因组复制事件,与拟南芥GRAS基因之间预测到16个共线性基因对。GRAS基因家族成员的表达具有一定的组织特异性,其启动子含有多个与生长发育、信号转导和胁迫相关的顺式作用元件,且部分成员响应盐和干旱胁迫,暗示其可能参与烟草胁迫响应等信号传导过程。本研究将为深入研究烟草GRAS家族基因的生物学功能奠定理论基础。GRAS proteins are a plant-specific protein family that play essential roles in various physiological processes,including plant growth,development and responses to biotic and abiotic stresses.In this study,95 tobacco GRAS genes were identified by using comparative genomics and bioinformatics.Of these,50 GRAS family members were unevenly distributed across 20 chromosomes.Phylogenetic analysis,collinearity analysis,and expression pattern examination were performed on these tobacco GRAS genes.Results showed that the newly identified GRAS genes from tobacco and Arabidopsis could be divided into eight subgroups.A total of26 GRAS members originated from genome-wide replication events,with 16 covariance pairs predicted to be associated with Arabidopsis thaliana GRAS genes.The GRAS genes promoters contain numerous development-and stress-related cis-elements.The expression of these genes showed tissue specificity,and some GRAS members exhibited altered expression levels under drought and salt stress treatments,suggesting their involvement in the signal transduction pathways associated with tobacco's stress responses.

关 键 词：烟草 GRAS 基因复制 表达模式 非生物胁迫 

分 类 号：S572[农业科学—烟草工业]