HPLC法检测化妆品中磺胺嘧啶和甲氧苄啶的研究  

Detection of Sulfadiazine and Timethoprim by HPLC in Cosmetics

作　　者：贠晓斐 张艳霞 韩锋 钱钰娟 孙颖 刘开玉 YUN Xiao-fei;ZHANG Yan-xia;HAN Feng;QIAN Yu-juan;SUN Ying;LIU Kai-yu(Beijing Chaoyang District Food and Drug Safety Monitoring Center,Beijing 100124,China)

机构地区：[1]北京市朝阳区食品药品安全监控中心,北京100124

出　　处：《广州化工》2024年第17期79-82,共4页GuangZhou Chemical Industry

摘　　要：方法色谱条件是以phenomenex Gemini C18柱(5μm,4.6 mm×250 mm)分离,以乙腈-0.3%醋酸铵溶液(15∶85,V/V)为流动相,流速为1.0 mL·min^(-1),二极管阵列检测器检测波长为240 nm。方法标准曲线相关系数均在0.999以上,线性关系良好。方法回收率在91%~104%之间。阳性样品的精密度及稳定性结果的相对标准偏差均在4%以内,稳定性较好。方法操作简单,具有较高的灵敏度、准确度,可用于同时定性与定量测定化妆品中两种抗生素磺胺嘧啶与甲氧苄啶。Two kinds of antibiotics were separated on phenomenex Gemini C 18 column(5μm,4.6 mm×250 mm),by elution with acetonitrile-0.3%ammonium acetate solution(15∶85,V/V).The flow rate was 1.0 mL·min^(-1).The DAD detection wavelength was 240 nm.The methods achieved good linearity in the ranges.The average recovery was 91%~104%,RSD was below 4%.The method had good precision and stability.The method was reliable,can be used for simultaneous determination of sulfadiazine and trimethoprim in cosmetics products.

关 键 词：抗生素 磺胺嘧啶 甲氧苄啶 化妆品 高效液相色谱-二级线性阵列检测器法 

分 类 号：TQ658[化学工程—精细化工]