生物炭输入对脐橙园酸性土壤固氮细菌群落结构的影响  

Effects of Biochar Input on the Community Structure of Nitrogen-fixing Bacteria in Acid Soil in Navel Orange Orchard

作　　者：岳鹏鹏 林文梦 莫义妹 黄鹏 杨春艳 于鸿浩 YUE Pengpeng;LIN Wenmeng;MO Yimei

机构地区：[1]桂林医学院智能医学与生物技术学院,广西桂林541199

出　　处：《智慧农业导刊》2024年第19期18-21,共4页JOURNAL OF SMART AGRICULTURE

基　　金：国家自然科学基金项目(32260030);广西科技基地和人才专项(桂科AD20238057);广西大学生创新创业训练项目(202310601050、S202210601164)。

摘　　要：广西柑橘园酸性土壤分布广泛、氮转化具有特殊性,生物炭常用于该区土壤改良,然而生物炭输入对该土壤固氮微生物群落的影响尚不清楚。该研究以广西脐橙园酸性土壤固氮细菌群落为研究对象,通过高通量测序及生信分析发现该土壤中的固氮菌群落覆盖8门15纲28目39科50属的微生物。变形菌门在生物炭处理后相对丰度增加,OTU数目、Ace指数、Chao指数在生物炭输入量5%的组最高,但各组间差异不显著。PcoA分析显示生物炭输入显著改变土壤固氮细菌群落结构,且变化程度与生物炭输入量有关。土壤pH、NH_(4)^(+)-N含量、矿化率是影响脐橙园酸性土壤固氮细菌群落结构的主要环境因子,固氮细菌不同种群对环境因子的响应并不一致。Biochar is commonly used for soil amendment in citrus orchards across Guangxi,where acidic soils are prevalent and nitrogen transformation is characterized by its specificity.However,the impact of biochar application on the microbial communities involved in nitrogen fixation remains unclear.This study focused on the microbial communities of nitrogen-fixing bacteria in the acidic soils of navel orange orchards in Guangxi.Through high-throughput sequencing and bioinformatics analysis,it was found that the nitrogen-fixing microbial community in these soils encompasses 50 genera within 39 families,28 orders,15 classes,and 8 phyla.The relative abundance of Proteobacteria increased following biochar treatment,and the highest OTU numbers,Ace index,and Chao index were observed in the group with a 5%biochar input,although differences in diversity indices among groups were not significant.PcoA and NMDS analyses demonstrated that biochar application significantly altered the structure of the soil nitrogen-fixing bacterial community,with the degree of change being related to the amount of biochar added.Soil pH,NH4_(+)^(-)N content,and mineralization rate were identified as the main environmental factors influencing the structure of the nitrogen-fixing bacterial community in the acidic soils of navel orange orchards,with different populations of nitrogen-fixing bacteria responding differently to environmental factors.

关 键 词：脐橙 生物炭 NIFH 固氮菌群落 多样性 

分 类 号：S666[农业科学—果树学]