甘草抗炎活性质量标志物筛选  

Screening of anti-inflammatory activity Q-markers for Glycyrrhizae Radix et Rhizoma

作　　者：陈琴 郑文惠 白海英 宋平顺 杨平荣 杨志刚 CHEN Qin;ZHENG Wen-hui;BAI Hai-ying;SONG Ping-shun;YANG Ping-rong;YANG Zhi-gang(College of Pharmacy,Lanzhou University,Lanzhou 730030,China;Gansu Provincial Institute of Drug Control,Lanzhou 730070,China;Gansu Provincial Collaborative Innovation Center for Medicinal Materials in Longxi,Lanzhou 730030,China)

机构地区：[1]兰州大学药学院,甘肃兰州730030 [2]甘肃省药品检验研究院,甘肃兰州730070 [3]甘肃省陇药协同创新中心,甘肃兰州730030

出　　处：《中成药》2024年第9期2841-2850,共10页Chinese Traditional Patent Medicine

基　　金：国家重点研发计划政府间国际科技创新合作重点专项(2016YFE0129000);甘肃省药品监督管理局科研项目(2020GSMPA-KL11,2022GSMPA0010)。

摘　　要：目的筛选甘草抗炎活性质量标志物。方法脂多糖刺激RAW264.7巨噬细胞建立炎症模型,测定不同等级药材NO抑制率。建立UHPLC-QTOF-MS指纹图谱,鉴定化学成分,绘制热图,进行正交偏最小二乘判别分析。结果一等、二等药材NO抑制率高于统货(P<0.05,P<0.01)。47批药材指纹图谱中有211个共有峰,共鉴定出56个化合物,包括17个皂苷类、6个黄酮类、8个黄烷酮类、4个查尔酮类、12个异黄酮类、9个其他类。一等、二等药材中异黄酮类、香豆素类、山柰酚、甘草黄酮醇相对含量高于统货,野生品中黄烷酮类、查尔酮类、皂苷类相对含量高于栽培品。新甘草苷、异甘草苷、山柰酚、hedysarimcoumestan E、甘草皂苷C2、甘草芳香豆素、甘草新木脂素、甘草瑞酮为质量标志物。结论该方法稳定可靠,对甘草质量控制具有参考价值。AIM To screen anti-inflammatory activity Q-markers for Glycyrrhizae Radix et Rhizoma.METHODS Lipopolysaccharide was used for stimulating RAW264.7 macrophages to establish inflammatory model,after which the NO inhibitory rates of different grades of medicinal materials were determined.The UHPLC-QTOF-MS fingerprints were established,after which chemical constituents were identified,heatmap was drawn,and orthogonal partial least squares analysis was performed.RESULTS Grade 1 and grade 2 medicinal materials demonstrated higher NO inhibitory rates than gradeless and uniformly-priced goods(P<0.05,P<0.01).There were 211 common peaks in the fingerprints of 47 batches of medicinal materials,total 56 compounds were identified,containing 17 saponins,6 flavonoids,8 flavanones,4 chalcones,12 isoflavones and 9 other kinds.The relative contents of isoflavones,coumarins,kaempferol and licoflavonol in grade 1 and grade 2 medicinal materials were higher than those in gradeless and uniformly-priced goods,while the relative contents of flavanones,chalcones and saponins in wild products were higher than those in cultivated products.Neoliquiritin,isoliquiritin,kaempferol,hedysarimcoumestan E,licorice saponin C 2,licoarylcoumarin,glicoricone and liconeolignan were taken as Q-markers.CONCLUSION This stable and reliable method has a certain reference value for the quality control of Glycyrrhizae Radix et Rhizoma.

关 键 词：甘草 抗炎活性 质量标志物 RAW264.7巨噬细胞 UHPLC-QTOF-MS指纹图谱 正交偏最小二乘判别分析 

分 类 号：R282[医药卫生—中药学]