黄连配方颗粒与标准汤剂化学成分一致性研究  

Consistency of chemical constituents between formula granules and standard decoction of Coptidis Rhizoma

作　　者：张代亮 董新娜 史磊[1] 董晓弟 林永强 张荣飞 张京华 姚元成 张风超 曹桂云 孟兆青 ZHANG Dai-liang;DONG Xin-na;SHI Lei;DONG Xiao-di;LIN Yong-qiang;ZHANG Rong-fei;ZHANG Jing-hua;YAO Yuan-cheng;ZHANG Feng-chao;CAO Gui-yun;MENG Zhao-qing(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Hongjitang Pharmaceutical Group Co.,Ltd.,Jinan 250103,China;Shandong Provincial Institute for Food and Drug Control,Jinan 250101,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]山东宏济堂制药集团股份有限公司,山东济南250103 [3]山东省食品药品检验研究院,山东济南250101

出　　处：《中成药》2024年第9期2851-2858,共8页Chinese Traditional Patent Medicine

基　　金：山东省重点研发计划重大科技创新工程项目(2021CXGC010511);泉城“5150”引才倍增计划项目(2021)。

摘　　要：目的考察黄连配方颗粒与标准汤剂化学成分的一致性。方法制备18批标准汤剂,测定出膏率及木兰花碱、药根碱、非洲防己碱、表小檗碱、黄连碱、巴马汀、小檗碱含量和转移率,建立HPLC特征图谱,进行聚类分析。结果18批标准汤剂和24批配方颗粒特征图谱中有11个共有峰,相似度0.861~1.000,聚为2类。配方颗粒出膏率及指标成分含量和转移率均与标准汤剂接近。结论黄连配方颗粒与标准汤剂化学成分的一致性良好,可为前者质量控制、工艺研究及临床应用提供参考。AIM To investigate the consistency of chemical constituents between formula granules and standard decoction of Coptidis Rhizoma.METHODS Eighteen batches of standard decoctions were prepared,after which the extraction rate and contents,transfer rates of magnolflorine,jatrorrhizine,columbamine,epiberberine,coptisine,palmatine,berberin were determined,HPLC characteristic chromatograms were established.RESULTS There were 11 common peaks in the characteristic chromatograms of 18 batches of standard decoctions and 24 batches of formula granules with the similarities of 0.861-1.000,which were clusterd into two categories.The formula granules and standard decoction demonstrated approximated extraction rate and contents,transfer rates of index constituents.CONCLUSION The chemical constituents between formula granules and standard decoction of Coptidis Rhizoma display good consistency,which can provide references for the quality control,process research and clinical application of the former.

关 键 词：黄连 配方颗粒 标准汤剂 HPLC特征图谱 聚类分析 

分 类 号：R927.2[医药卫生—药学]