UHPLC Q-Exactive Plus Orbitrap HRMS法鉴定隔山消提取物在功能性消化不良大鼠体内代谢产物  

Identification of in vivo metabolites of Cynanchum auriculatum extract in functional dyspepsia rats by UHPLC Q-Exactive Plus Orbitrap HRMS

作　　者：吴宗芹 勾健 李勇军[3] 陆苑[1] 冉巧巧 孙佳[1] WU Zong-qin;GOU Jian;LI Yong-jun;LU Yuan;RAN Qiao-qiao;SUN Jia(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,China;School of Pharmacy,Guizhou Medical University,Guiyang 550004,China;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Ministry of Education,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学省部共建药用植物功效与利用国家重点实验室/贵州省药物制剂重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550004 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004

出　　处：《中成药》2024年第9期2876-2884,共9页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82260811);贵州医科大学博士启动基金项目(校博合J字[2020]013号)。

摘　　要：目的建立UHPLC Q-Exactive Plus Orbitrap HRMS法鉴定隔山消提取物在功能性消化不良大鼠体内代谢产物。方法建立功能性消化不良大鼠模型。分析采用Hypersi 1 GOLD C_(18)色谱柱(2.1 mm×100 mm,1.9μm);流动相水(含0.1%甲酸)-乙腈(含0.1%甲酸),梯度洗脱;体积流量0.3 mL/min;柱温40℃;电喷雾离子源;正负离子扫描。结果共鉴定出4种原型成分(白首乌二苯酮、去酰基萝藦苷元、青阳参苷元、丁香酸)及其110种代谢产物,其中粪便、尿液共有代谢产物12种,尿液特有代谢产物56种,粪便特有代谢产物42种。原型成分代谢途径包括Ⅰ相代谢(氧化、还原等)、Ⅱ相代谢(硫酸化、葡萄糖醛酸化等)及Ⅰ相、Ⅱ相复合反应。结论该方法高效全面,可为进一步寻找隔山消潜在活性代谢产物奠定基础。AIM To identify the in vivo metabolites of Cynanchum auriculatum Royle ex Wight extract in functional dyspepsia rats by UHPLC Q-Exactive Plus Orbitrap HRMS.METHODS The rat models for functional dyspepsia were established.The analysis was performed on a 40℃thermostatic Hypersil GOLD C_(18)column(2.1 mm×100 mm,1.9μm),with the mobile phase comprising of water(containing 0.1%formic acid)-acetonitrile(containing 0.1%formic acid)flowing at 0.3 mL/min in a gradient elution manner,and electrospray ionization source was adopted in positive and negative ion scanning.RESULTS Total 4 prototypes(baishouwubenzophenone,deacylmetaplexigenin,qingyangshengenin,syringic)and 110 metabolites were identified,12 of which were common metabolites in feces and urine,56 of which were unique metabolites in urine,42 of which were unique metabolites in feces.The metabolic pathway of prototypes contained phaseⅠmetabolism(reduction,oxidization,etc.),phaseⅡmetabolism(sulfonation,glucuronidation,etc.)and phaseⅠ,Ⅱcomposite reactions.CONCLUSION This effective and comprehensive method can lay the theoretical foundation for further discovery of potential active metabolites in C.auriculatum.

关 键 词：隔山消提取物 代谢产物 功能性消化不良 UHPLC Q-Exactive Plus Orbitrap HRMS 

分 类 号：R969.1[医药卫生—药理学]