抵当汤含药血清对高糖诱导大鼠肾小球内皮细胞损伤的影响  

Effects of Didang Decoction-containing serum on high glucose-induced injury of rat glomerular endothelial cells

作　　者：罗宝璐 储全根[1,2] 储俊[1,2] 李飞翔 陈静[1] 王悦琦 LUO Bao-lu;CHU Quan-gen;CHU Jun;LI Fei-xiang;CHEN Jing;WANG Yue-qi(College of Traditional Chinese Medicine,Anhui University of Chinese Medicine,Hefei 230012,China;Ministry of Education Key Laboratory for Xin'an Medicine,Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]安徽中医药大学中医学院,安徽合肥230012 [2]安徽中医药大学新安医学教育部重点实验室,安徽合肥230012

出　　处：《中成药》2024年第9期2930-2935,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(8177152144);安徽省中医药领军人才项目(ZYYLJRC201911)。

摘　　要：目的观察抵当汤含药血清对高糖诱导损伤大鼠肾小球内皮细胞(RGECs)的保护作用。方法大鼠灌胃给予蒸馏水或抵当汤制备空白血清或抵当汤含药血清,CCK8法筛选葡萄糖造模浓度及含药血清给药浓度。将RGECs分为空白组、模型组、抵当汤组(10%抵当汤含药血清)、达格列净组(空白血清+2μmol/L达格列净)、抵当汤+达格列净组(10%抵当汤含药血清+2μmol/L达格列净),药物处理24 h后,RT-qPCR法和Western blot法检测细胞Nrf2、HO-1、NQO-1 mRNA和蛋白表达,免疫荧光法检测细胞Nrf2荧光表达,比色法检测细胞SOD活性及MDA水平。结果与空白组比较,模型组RGECs中Nrf2、HO-1、NQO-1 mRNA和蛋白表达以及SOD活性降低(P<0.01),MDA水平升高(P<0.01);与模型组比较,各给药组Nrf2、HO-1、NQO-1 mRNA和蛋白表达以及SOD活性升高(P<0.05,P<0.01),MDA水平降低(P<0.01)。结论抵当汤含药血清可以通过激活Nrf2信号通路从而抑制氧化应激,对高糖损伤的RGECs起到保护作用。AIM To investigate the protective effects of Didang Decoction-containing serum on rat glomerular endothelial cells(RGECs)following high glucose injury.METHODS Rats were given distilled water or Didang Decoction by gavage to prepare the blank serum or Didang Decoction-containing serum.CCK8 method was used to screen the glucose concentration for the modeling and serum concentration of the drug.The RGECs were divided into the blank group,the model group,Didang Decoction group(10%Didang Decoction medicated serum),Dapagliflozin group(normal serum+2μmol/L Dapagliflozin)and Didang Decoction+Dapagliflozin group(10%Didang Decoction medicated serum+2μmol/L Dapagliflozin).24 hrs after the drug treatment,the RGECs had their mRNA and protein expressions of Nrf2,HO-1 and NQO-1 detected by RT-qPCR method and Western blot method;their Nrf2 fluorescence expression detected by immunofluorescence method;and their SOD activity and MDA level detected by colorimetric method.RESULTS Compared with the blank group,the model group displayed decreased mRNA and protein expressions of Nrf2,HO-1 and NQO-1 as well as the activity of SOD(P<0.01),and increased MDA level(P<0.01).Compared with the model group,the groups intervened with the drugs showed increased mRNA and protein expressions of Nrf2,HO-1 and NQO-1 as well as the activity of SOD(P<0.05,P<0.01),and decreased MDA level(P<0.01).CONCLUSION Didang Decoction-containing serum can protect RGECs from high glucose injury through activating the Nrf2 signaling pathway.

关 键 词：抵当汤 大鼠肾小球内皮细胞(RGECs) 糖尿病肾病 Nrf2信号通路 氧化应激 

分 类 号：R285.5[医药卫生—中药学]