基于网络药理学探讨益艾康胶囊治疗艾滋病的作用机制  

Mechanism of Yiaikang Capsule in the treatment of AIDS based on network pharmacology

作　　者：杨琴 彭鑫 杨欣怡 刘晶晶[2] 王玉璐 兰燕玲 苗丁山 苏琛[2] YANG Qin;PENG Xin;YANG Xinyi;LIU Jingjing;WANG Yulu;LAN Yanling;MIAO Dingshan;SU Chen(Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan,China;Sichuan Academy of Traditional Chinese Medicine,Chengdu 610041,Sichuan,China)

机构地区：[1]成都中医药大学,成都610075 [2]四川省中医药科学院,成都610041

出　　处：《中国艾滋病性病》2024年第8期826-831,共6页Chinese Journal of Aids & STD

基　　金：四川省中医药管理局科学技术研究专项(2023MS224)。

摘　　要：目的基于网络药理学分析益艾康胶囊治疗艾滋病的靶点和信号通路探讨其潜在的作用机制。方法利用中药系统药理学分析平台数据库检索益艾康胶囊相关药物化学成分及其对应的靶点,检索GeneCards、OMIM和NCBI数据库中艾滋病的潜在靶点,应用Excel软件获取益艾康胶囊-艾滋病交集靶点,利用Cytoscape软件和STRING数据库平台构建蛋白互作网络图,并进行拓扑学分析,通过DAVID数据库平台对益艾康胶囊治疗艾滋病的生物学过程及代谢通路进行GO富集和KEGG富集分析。结果通过网络药理学分析,获取益艾康胶囊的化学成分有90个,对应作用靶点有229个。与艾滋病相关的化学成分有81种,其中包括但不限于槲皮素、山柰酚、汉黄芩素、黄芩素以及β-谷甾醇等关键物质。与艾滋病相关联的靶点有110个,其中较为显著的有AKT1以及细胞因子IL-6、TNF、TP53、CASP3。益艾康胶囊可能参与艾滋病治疗的生物学过程为凋亡过程负调控、基因表达的正调控、RNA聚合酶Ⅱ启动子的转录正调控、酶结合、蛋白结合、蛋白激酶结合等,可能通过调节IL-17信号通路、TNF信号通路和PI3KAKT信号通路等发挥治疗艾滋病的作用。结论本研究初步探索了益艾康胶囊治疗艾滋病的化学成分、靶点通路及相互作用关系。预测益艾康胶囊治疗艾滋病主要通过调控TNF、AKT1基因的表达干预TNF通路和PI3K-AKT通路发挥作用。Objective This study aimed to explore the potential mechanisms of action of the Yiaikang Capsule in treating AIDS by analyzing its target proteins and signaling pathways using network pharmacology.Methods The Traditional Chinese Medicine Systems Pharmacology(TCMSP)database was used to retrieve the chemical components and corresponding targets of Yiaikang Capsules.Potential targets related to AIDS were obtained from GeneCards,Online Mendelian Inheritance in Man(OMIM),and National Center for Biotechnology Information(NCBI)Databases.The intersection targets between the Yiaikang Capsule and AIDS were obtained using Excel.A protein-protein interaction network was constructed using Cytoscape software and the STRING database platform,followed by topological analysis.GO enrichment and KEGG pathway analyses of the biological processes and metabolic pathways related to the Yiaikang Capsule for treating AIDS were performed using the DAVID database platform.Results Network pharmacology analysis identified 90 chemical components and 229 corresponding targets in the Yiaikang Capsule.There are 81 chemical components related to AIDS,including but not limited to quercetin,kaempferol,wogonin,baicalein,β-sitosterol and other key substances.There are 110 targets associated with AIDS,among which AKT1 and cytokines IL-6,TNF,TP53,CASP3are more significant.Yiaikang Capsule may treat AIDS through biological processes,such as the negative regulation of the apoptotic process,positive regulation of gene expression,positive regulation of RNA polymeraseⅡpromoter transcription,enzyme binding,protein binding,and protein kinase binding.Its therapeutic effects may be exerted on AIDS by modulating signaling pathways,such as IL-17,TNF,and PI3KAKT.Conclusions This study provides a preliminaryⅡexploration of the chemical components,target pathways,and interaction relationships of the Yiaikang Capsule in AIDS treatment.It is predicted that the Yiaikang Capsule may intervene in the TNF and PI3K-AKT pathways by regulating the expression of TNF and

关 键 词：益艾康胶囊 艾滋病 作用机制 网络药理学 

分 类 号：R512.91[医药卫生—内科学]