基于AMPK/SIRT1/PGC-1α通路的鹿鞭四气表征研究  

Study on four natures characterization of Lubian based on AMPK/SIRT1/PGC-1αpathway

作　　者：林清 陶然 梁唯灿 李一芃 陈亚文 吴范武 王林元[3] LIN Qing;TAO Ran;LIANG Weican;LI Yipeng;CHEN Yawen;WU Fanwu;WANG Linyuan(College of Traditional Chinese Medicine,North China University of Science and Technology,Tangshan 063210,China;School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102401,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102401,China)

机构地区：[1]华北理工大学中医学院,唐山063210 [2]北京中医药大学中医学院,北京102401 [3]北京中医药大学中药学院,北京102401

出　　处：《中华中医药杂志》2024年第8期4017-4024,共8页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：科技部重点研发计划中医药现代化重点专项(No.2018YFC1706801)。

摘　　要：目的:研究鹿鞭的药性表征,探讨其四气归属。方法:将健康雄性昆明小鼠88只适应性喂养1周后随机分为11组:空白组,虚寒模型组,人参组,虚寒鹿鞭低、中、高剂量组,虚热模型组,西洋参组,虚热鹿鞭低、中、高剂量组,每组8只。每天下午分别定时灌胃氢化可的松、醋酸地塞米松制备虚寒、虚热模型,每天上午定时给予相应药物,空白组给予空白溶剂,连续14 d。于第14天给药后30 min测定各组小鼠力竭游泳时间;于第15天摘眼球取血分离血清测定血清中乳酸(LA)、尿素氮(BUN)、总胆固醇(TC)、甘油三酯(TG)含量,剖取大腿骨骼肌测定肌糖原含量、肌肉组织中Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶活性以及P-AMPK、AMPK、SIRT1、PGC-1α的蛋白表达情况。结果:与虚寒模型组比较,虚寒鹿鞭低、中剂量组体质量显著升高,虚寒症状有所改善,力竭游泳时间显著增加,血LA含量显著降低,BUN、TC及肌糖原含量显著增加,肌肉Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶活性显著升高,肌肉组织P-AMPK/AMPK值、SIRT1、PGC-1α含量明显增加。与虚热模型组比较,虚热鹿鞭低、中剂量组体质量显著升高,虚热症状有所改善,力竭游泳时间显著增加,血LA、BUN含量显著降低,肌糖原含量显著增加,肌肉Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶活性显著降低,肌肉组织P-AMPK/AMPK值、SIRT1、PGC-1α含量显著降低。结论:鹿鞭可以通过影响AMPK/SIRT1/PGC-1α通路调节肌肉组织能量代谢异常状态,进而双向调节小鼠虚寒及虚热状态,增强虚寒、虚热小鼠体力,故鹿鞭四气属平。Objective:To study the pharmacological characterization of Lubian and explore its four qi attribution.Methods:Eighty-eight healthy male Kunming mice were randomly divided into blank group,deficiency cold model group,ginseng group,deficiency cold plus low,medium and high dose Lubian groups,deficiency heat model group,American ginseng group,and deficiency heat plus low,medium and high dose Lubian groups after 1 week of adaptive feeding.Each group had 8mice,totaling 11 groups.Each afternoon,hydrocortisone and dexamethasone acetate were administered by gavage to prepare models of deficiency cold and deficiency heat.The corresponding drugs were given at regular intervals every morning,while the blank group was given blank solvent for 14 consecutive days.Measure the exhaustion swimming time of mice in each group 30 minutes after administration on the 14th day;On the 15th day,blood was extracted from eyeballs and serum was separated to determine the levels of blood lactate(LA),urea nitrogen(BUN),total cholesterol(TC),triglycerides(TG)in the serum.Thigh skeletal muscles were dissected to determine muscle glycogen levels Na^(+)-K^(+)-ATPase,Ca^(2+)-Mg^(2+)-ATPase activity,as well as the protein expression status of P-AMPK,AMPK,SIRT1,PGC-1αin muscle tissue.Results:Compared with the deficiency cold model group,the deficiency cold plus low,medium dose Lubian groups showed a significant increase in body mass,improved deficiency cold symptoms,significantly increased exhaustion swimming time,significantly decreased blood LA content,significantly increased BUN,TC and muscle glycogen content,and significantly increased muscle Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase activities,muscle tissue P-AMPK/AMPK values,SIRT1,PGC-1αcontent significantly increased.Compared with the deficiency heat model group,the deficiency heat plus low,medium dose Lubian groups showed a significant increase in body mass,improvement in deficiency heat symptoms,a significant increase in exhausted swimming time,a significant decrease in blood LA and B

关 键 词：鹿鞭 药性 性平 抗疲劳 能量代谢 AMPK通路 

分 类 号：R285[医药卫生—中药学]