补肾降压方对盐敏感性高血压大鼠肾损害及ACE2/Ang(1-7)/Mas轴的影响  

Effects of Bushen Jiangya Prescription on renal damage and ACE2/Ang(1-7)/Mas axis in salt-sensitive hypertensive rats

作　　者：刘巍[1] 熊兴江[2] 刘红旭[1] 王阶[2] 褚福永[1] LIU Wei;XIONG Xingjiang;LIU Hongxu;WANG Jie;CHU Fuyong(Department of Cardiology,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;Department of Cardiology,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]首都医科大学附属北京中医医院心血管科,北京100010 [2]中国中医科学院广安门医院心血管科,北京100053

出　　处：《中华中医药杂志》2024年第8期4317-4321,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82204997);国家中医药管理局中医药循证能力建设项目(No.2019XZZX-XXG001);第六批北京市级中医药专家学术经验继承工作项目;首都医科大学附属北京中医医院中医药学科建设-中医心病学。

摘　　要：目的:探讨补肾降压方对盐敏感性高血压大鼠(DS)血管紧张素转换酶(ACE2)/血管紧张素(Ang1-7)/Mas轴的调节作用,探讨其对高血压肾损害的治疗作用机制。方法:选用DS大鼠48只,随机分为低盐(LS)组、高盐(HS)组、缬沙坦(Va)组和补肾降压(BS)组,每组12只,LS组喂以0.4%低盐饲料,其余3组喂以8%高盐饲料,不同饲料喂养3周后每日按照2 mL/100 g灌胃,连续给药8周。给药前后测量收缩压,酶法检测大鼠尿微量白蛋白(mAlb)、β2-微球蛋白(β2-MG)及24 h尿蛋白定量水平及血浆中AngⅡ、Ang(1-7)的含量,苏木素-伊红(HE)染色肾脏组织,RT-PCR法及Western Blot法分别检测肾脏ACE2、Mas mRNA及蛋白表达。结果:给药后,与LS组比较,其余各组收缩压均明显升高,尿mAlb、尿β2-MG及24 h尿蛋白定量升高,血浆AngⅡ升高,Ang(1-7)降低,肾脏ACE2、Mas蛋白及mRNA表达显著降低(P<0.05,P<0.01)。与HS组比较,Va及BS组各时间点收缩压降低,尿mAlb、尿β2-MG及24h尿蛋白定量降低,血浆AngⅡ降低,Ang(1-7)升高、肾脏ACE2、Mas蛋白及mRNA表达升高(P<0.05,P<0.01)。结论:补肾降压方平稳降压,改善尿蛋白的作用,其作用机制可能与调节ACE2/Ang(1-7)/Mas轴从而拮抗肾素-血管紧张素-醛固酮系统(RAAS)系统活性相关。Objective:To investigate the effects of Bushen Jiangya Prescription on angiotensin converting enzyme(ACE2)/angiotensin(Ang)(1-7)/Mas axis in Dahl salt-sensitve rats(DS),and to explore the mechanism of its prevention and treatment of renal damage in hypertension.Methods:Forty-eight DS rats were randomly divided into low salt(LS)group,high salt(HS)group,valsartan(Va)group and Bushen Jiangya(BS)group,with 12 rats in each group.LS group was fed with 0.4%low salt diet,HS group,Va group and BS group were fed with 8%high salt diet.After 3 weeks of different diets,2 mL/100 g was given intragastric administration for 8 consecutive weeks.Blood pressure was measured once before administration.Urine microalbumin(mAlb),β2-microglobulin(β2-MG)and 24h urine protein were detected by enzyme method,plasma AngⅡ,Ang(1-7)content were detected by ELISA method,kidney tissue was stained with hematoxylin-eosin(HE).The mRNA expressions of ACE2 and Mas in kidney were detected by RT-PCR,and the protein expressions of ACE2 and Mas in kidney were detected by Western Blot.Results:After administration,compared with LS group,systolic blood pressure,urine mAlb,urineβ2 and 24h urine protein were increased,plasma AngⅡlevel was increased,Ang(1-7)level was decreased,ACE2,Mas protein and mRNA expression were decreased(P<0.05,P<0.01).Compared with HS group,Va and BS groups decreased systolic blood pressure,urine mAlb,urineβ2 and 24h urine protein,plasma AngⅡlevel decreased,plasma Ang(1-7)level increased,ACE2,Mas protein and mRNA expression in kidney increased(P<0.05,P<0.01).Conclusion:Bushenjiangya Prescription can stabilize blood pressure and improve urinary protein,and its mechanism may be related to antagonizing the activity of renin-angiotensin-aldosterone system(RAAS)by regulating ACE2/Ang(1-7)/Mas axis.

关 键 词：盐敏感性高血压 补肾降压方 高血压肾损害 血管紧张素转换酶2/血管紧张素(1-7)/Mas轴 

分 类 号：R285.5[医药卫生—中药学]