2019-2020年河北省百日咳鲍特菌分离株基因多样性分析  

Genetic diversity of Bordetella pertussis strains isolated during 2019-2020 in Hebei province

作　　者：贾肇一[1] 郑飞 张洪斌 王乐雨 张海霞[1] 张文超[3] 段素霞 王若璇 孙印旗[1] 何宝花[1] Jia Zhaoyi;Zheng Fei;Zhang Hongbin;Wang Leyu;Zhang Haixia;Zhang Wenchao;Duan Suxia;Wang Ruoxuan;Sun Yinqi;He Baohua(Hebei Provincial Center for Disease Control and Prevention,Hebei Key Laboratory of Pathogens and Epidemiology of Infectious Diseases,Shijiazhuang 050021,Hebei,China;School of Public Health,North China University of Science and Technology,Tangshan 063210,Hebei,China;Hebei Children's Hospital,Shijiazhuang 050000,Hebei,China)

机构地区：[1]河北省疾病预防控制中心,河北省传染病病原鉴定分析与流行病学重点实验室,河北石家庄050021 [2]华北理工大学公共卫生学院,河北唐山063210 [3]河北省儿童医院,河北石家庄050000

出　　处：《中国疫苗和免疫》2024年第4期415-420,共6页Chinese Journal of Vaccines and Immunization

基　　金：河北省医学科学研究课题计划(20210177)。

摘　　要：目的分析河北省百日咳鲍特菌(Bordetella pertussis,Bp)分离株基因多样性。方法对2019-2020年河北省儿童医院分离的Bp流行株进行二代全基因组测序和组装拼接获得全基因组序列,进行多位点序列分型(Multilocus sequence typing,MLST)、多位点抗原序列分型(Multilocus antigen sequence typing,MAST)、多级基因组分型(Multilevel genome typing,MGT)、Bp疫苗抗原序列分型(Bordetella pertussis vaccine antigens sequence typing,BPagST)和23S rRNA基因突变分析。结果在62株Bp分离株中,MLST均为ST-2型,56株携带ptxP1基因的分离株与疫苗株相比发生23S rRNA A2047G突变(其中6株属于Prn基因缺陷型),6株携带ptxP3基因的Bp菌株未发生该突变;MAST包括6种型别,主要为prn1/ptxP1/ptxA1/fim3-1/fim2-1(47株);MGT在MGT1至MGT5水平上分别包括1种、2种、3种、14种和52种型别;BPagST包括BPagST-37型(56株)和BPagST-4型(6株)。结论本研究Bp分离株与疫苗株相比主要毒力蛋白基因和核心基因组发生明显变化,可能导致BP流行株发生疫苗免疫逃逸。需进一步加强Bp流行株基因组监测。Objective To explore the genetic diversity of Bordetella pertussis(Bp)strains isolated in Hebei province.Methods We selected Bp isolates from 2019 to 2020 in Hebei Children′s Hospital for next generation sequencing.We assembled and spliced sequences to obtain whole genomes for multilocus sequence typing(MLST),multilocus antigen sequence typing(MAST),multilevel genome typing(MGT),Bp vaccine antigen typing(BPagST),and 23S rRNA gene mutation analyses.Results A total of 62 Bp strains were obtained;all were MLST type ST-2,among which 56 carrying the ptxP1 gene had 23S rRNA gene A2047G mutations compared with vaccine strains(6 had Prn gene deletion),and 6 strains carrying the ptxP3 gene did not have mutations.Six MAST types were detected,among which the type prn1/ptxP1/ptxA1/fim3-1/fim2-1 was detected in 47 strains.There were 1,2,3,14,and 52 MGT types from MGT1 to MGT5 levels,respectively.BPagST types included BPagST-37(56 strains)and BPagST-4(56 strains).Conclusions The main virulence protein genes and core genomes of Bp isolates were significantly changed compared with vaccine strains,possibly leading to immune escape of circulating BP strains from vaccination.We suggest strengthening Bp genome monitoring.

关 键 词：百日咳 百日咳鲍特菌 基因型 多位点序列分型 多位点抗原序列分型 多级基因组分型 百日咳鲍特菌疫苗抗原序列分型 

分 类 号：R183[医药卫生—流行病学] R183.3[医药卫生—公共卫生与预防医学]