Overexpression of tonoplast Ca^(2+)-ATPase in guard cells synergistically enhances stomatal opening and drought tolerance  

作　　者：Jinghan Su Bingqing He Peiyuan Li Baiyang Yu Qiwen Cen Lingfeng Xia Yi Jing Feibo Wu Rucha Karnik Dawei Xue Michael R.Blatt Yizhou Wang 

机构地区：[1]Institute of Crop Science,Zhejiang University,Hangzhou 310058,China [2]Laboratory of Plant Physiology and Biophysics,University of Glasgow,Glasgow G128QQ,UK [3]BGI Research,Sanya 572025,China [4]College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China [5]Zhejiang Provincial Key Laboratory of Crop Germplasm,Zhejiang University,Hangzhou 310058,China

出　　处：《Journal of Integrative Plant Biology》2024年第8期1587-1602,共16页植物学报（英文版）

基　　金：supported by the Zhejiang Provincial Natural Science Foundation(LR21C020001);the National Natural Science Foundation of China(32372017,31871537 and U2003115);the Hainan Seed Industry Laboratory(B21HJ0220)to Y.W.;the Biotechnology and Biological Sciences Research Council(BBSRC)grants(BB/W001217/1)to M.R.B.and R.K.;BBSRC grants(BB/S017348/1);Royal Society University Research Fellowship awards(URFR 211002)to R.K.

摘　　要：Stomata play a crucial role in plants by controlling water status and responding to drought stress.However,simultaneously improving stomatal opening and drought tolerance has proven to be a significant challenge.To address this issue,we employed the OnGuard quantitative model,which accurately represents the mechanics and coordination of ion transporters in guard cells.With the guidance of OnGuard,we successfully engineered plants that overexpressed the main tonoplast Ca^(2+)-ATPase gene,ACA11,which promotes stomatal opening and enhances plant growth.Surprisingly,these transgenic plants also exhibited improved drought tolerance due to reduced water loss through their stomata.Again,OnGuard assisted us in understanding the mechanism behind the unexpected stomatal behaviors observed in the ACA11 overexpressing plants.Our study revealed that the overexpression of ACA11 facilitated the accumulation of Ca^(2+)in the vacuole,thereby influencing Ca^(2+)storage and leading to an enhanced Ca^(2+)elevation in response to abscisic acid.This regulatory cascade finely tunes stomatal responses,ultimately leading to enhanced drought tolerance.Our findings underscore the importance of tonoplast Ca^(2+)-ATPase in manipulating stomatal behavior and improving drought tolerance.Furthermore,these results highlight the diverse functions of tonoplast-localized ACA11 in response to different conditions,emphasizing its potential for future applications in plant enhancement.

关 键 词：ACA11 DROUGHT guard cells modeling STOMATA 

分 类 号：Q945[生物学—植物学]