The AaBBX21–AaHY5 module mediates light-regulated artemisinin biosynthesis in Artemisia annua L.  

作　　者：Weizhi He Hang Liu Zhangkuanyu Wu Qing Miao Xinyi Hu Xin Yan Hangyu Wen Yaojie Zhang Xueqing Fu Li Ren Kexuan Tang Ling Li 

机构地区：[1]Frontiers Science Center for Transformative Molecules,Joint International Research,Laboratory of Metabolic and Developmental Sciences,Key Laboratory of Urban Agriculture(South)Ministry of Agriculture,Plant Biotechnology Research Center,Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [2]Institute for Agri-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China

出　　处：《Journal of Integrative Plant Biology》2024年第8期1735-1751,共17页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (32070329, 31770327);Shanghai Natural Science Foundation (16ZR1418000);the National Key R&D Program of China (2018Y FA0900600)。

摘　　要：The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood(Artemisia annua L.).Light was previously shown to promote artemisinin production,but the underlying regulatory mechanism remains elusive.In this study,we demonstrate that ELONGATED HYPOCOTYL5(HY5),a central transcription factor in the light signaling pathway,cannot promote artemisinin biosynthesis on its own,as the binding of AaHY5 to the promoters of artemisinin biosynthetic genes failed to activate their transcription.Transcriptome analysis and yeast two-hybrid screening revealed the B-box transcription factor AaBBX21 as a potential interactor with AaHY5.AaBBX21 showed a trichome-specific expression pattern.Additionally,the AaBBX21–AaHY5 complex cooperatively activated transcription from the promoters of the downstream genes AaGSW1,AaMYB108,and AaORA,encoding positive regulators of artemisinin biosynthesis.Moreover,AaHY5 and AaBBX21 physically interacted with the A.annua E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1(COP1).In the dark,AaCOP1 decreased the accumulation of AaHY5 and AaBBX21 and repressed the activation of genes downstream of the AaHY5–AaBBX21 complex,explaining the enhanced production of artemisinin upon light exposure.Our study provides insights into the central regulatory mechanism by which light governs terpenoid biosynthesis in the plant kingdom.

关 键 词：AaBBX21 AaHY5 AaCOP1 Artemisia annua L. artemisinin biosynthesis LIGHT 

分 类 号：S567.219[农业科学—中草药栽培]