8例HIV抗体不确定病例随访及基因分型分析  

Analysis of genotype identification and follow-up of 8 cases with uncertain detection of HIV antibody

作　　者：白雪[1] 陈思佳 何丽娟 米娜瓦尔·艾拜[1] 孙磊[1] 杨雪蔚 BAI Xue;CHEN Si-jia;HE Li-juan;Minawaer AIBAI;SUN Lei;YANG Xue-wei(Department of STD and AIDS Control and Prevention,Urumuqi Municipal Center for Disease Control and Prevention,Urumqi,Xinjiang 830026,China)

机构地区：[1]乌鲁木齐市疾病预防控制中心性病艾滋病防制科,乌鲁木齐830026

出　　处：《疾病预防控制通报》2024年第4期57-61,F0004,共6页Bulletin of Disease Control & Prevention(China)

基　　金：新疆艾滋病防控研究重点实验室2021年度开放课题(XJYS1706-2021003)。

摘　　要：目的分析如何从检测策略上最大限度地发现艾滋病病毒(HIV)感染者,尽早发现HIV早期感染者,减少HIV的传播。方法对8例确证试验不确定的HIV感染者进行各项检测,结合其流行病史分析判断HIV感染状况。结果8份样本对第三代抗体筛查试验结果有反应或第四代抗原抗体筛查试验结果有反应,而免疫印记(western blot,WB)试验结果不确定。随访中,所有样本酶联免疫法试验OD值明显升高,WB检测结果阳转,其中5份病毒载量大于5000拷贝/ml。序列分析结果显示,8份HIV基因型均为CRF07_BC亚型,其中2份出现耐药突变。结论在开展HIV抗体筛查试验的基础上,采用抗原抗体检测试剂并结合病毒载量检测技术,可以有效提高HIV感染者的检出率;需要加强基因型及耐药株的监测。Objective To analyze how to maximize the detection of HIV infectors according to the detection strategies,and find the early HIV infectors as soon as possible,so as to reduce the transmission of HIV.Methods Various detection items were carried out for 8 HIV cases without confirmed test results,and the HIV infection status were analyzed and judged with epidemiological history.Results There were 8 samples reacted to the third generation antibody screening test or the fourth generation antigen and antibody screening test,but western blot(WB)test results were uncertain.During follow-up visits,the OD values of ELISA tests of all samples obviously increased that the OD values of ELISA increased and the WB test results changed to positive.Of which,the viral load of 5 samples were more than 5000 copies/ml.The results of sequence analysis showed the genotype of HIV were CRFO7_BC in 8 samples,and the drug-resistant mutation occurred in 2 samples.Conclusions Base on the HIV antibody screening tests,the HIV antigen and antibody diagnostic reagent,and the viral load test can effectively improve the detection rate of HIV infected patient.The surveillance of genotype and the strain with drug resistance(TDR)should be further strengthened.

关 键 词：HIV 病毒载量 基因亚型 

分 类 号：R521.91[医药卫生—内科学]