白藜芦醇对高碘诱导的甲状腺滤泡细胞内质网应激和凋亡的影响  

Effect of resveratrol on endoplasmic reticulum stress and apoptosis in thyroid follicular cells induced by high iodine

作　　者：张一斐 李书芹 杨志勇 ZHANG Yifei;LI Shuqin;YANG Zhiyong(Department of Oncology,Chongming Hospital Affiliated to Shanghai College of Health Medicine,Shanghai,202150;Department of Hematology,Chongming Hospital Affiliated to Shanghai College of Health Medicine,Shanghai,202150)

机构地区：[1]上海健康医学院附属崇明医院肿瘤科,上海202150 [2]上海健康医学院附属崇明医院血液内科,上海202150

出　　处：《实用临床医药杂志》2024年第16期44-48,共5页Journal of Clinical Medicine in Practice

基　　金：上海市崇明区可持续发展科技创新行动计划项目(CKY2021-09)。

摘　　要：目的探讨白藜芦醇对高碘诱导的甲状腺滤泡细胞内质网应激和凋亡的影响。方法体外培养甲状腺滤泡细胞系Nthy-ori 3-1,根据不同培养方法分为正常培养组(Normal组)、高碘组(HI组)、白藜芦醇组(RES组)和高碘联合白藜芦醇组(HI+RES组)。采用CCK-8法检测各组细胞增殖活性,采用TUNEL法检测各组细胞凋亡情况,采用实时荧光定量聚合酶链反应(PCR)检测各组细胞BIP、CHOP、IRE1 mRNA表达,采用蛋白质印迹法(Western blot)检测BIP、CHOP、IRE1蛋白表达。结果与Normal组相比,HI组细胞培养72 h后增殖活性下降,凋亡细胞占比升高,TUNEL染色阳性细胞占比升高,BIP、CHOP、IRE1 mRNA相对表达量升高,BIP、CHOP蛋白相对表达量升高,差异有统计学意义(P<0.001或P<0.05);与HI组相比,HI+RES组细胞培养72 h后增殖活性升高,凋亡细胞占比降低,TUNEL染色阳性细胞占比降低,BIP、CHOP mRNA相对表达量降低,BIP、CHOP蛋白相对表达量降低,差异有统计学意义(P<0.001或P<0.01)。结论白藜芦醇对高碘诱导的甲状腺滤泡细胞具有保护效应,可显著抑制碘过量摄入引起的内质网应激和细胞凋亡。Objective To investigate the effect of resveratrol on endoplasmic reticulum stress and apoptosis in thyroid follicular cells induced by high iodine.Methods The thyroid follicular cell line Nthy-ori 3-1 was cultured in vitro and divided into four groups based on different culture methods:normal culture group(Normal group),high iodine group(HI group),resveratrol group(RES group),and high iodine combined with resveratrol group(HI+RES group).Cell proliferation activity was detected by CCK-8 assay,cell apoptosis was detected by TUNEL assay,and the BIP,CHOP,IRE1 mRNA expressions were detected by real-time quantitative polymerase chain reaction(PCR).Western blot was used to detect the protein expressions of BIP,CHOP,and IRE1.Results Compared with the Normal group,the proliferation activity of cells in the HI group decreased after 72 hours of culture,the proportion of apoptotic cells increased,the proportion of TUNEL staining positive cells increased,and the relative expressions of BIP,CHOP,IRE1 mRNA,as well as BIP and CHOP proteins were significantly elevated(P<0.001 or P<0.05).Compared with the HI group,the proliferation activity of cells in the HI+RES group increased after 72 hours of culture,the proportion of apoptotic cells decreased,the proportion of TUNEL staining positive cells decreased,and the relative expressions of BIP,CHOP mRNA,as well as BIP and CHOP proteins,were significantly reduced(P<0.001 or P<0.01).Conclusion Resveratrol exerts a protective effect on thyroid follicular cells induced by high iodine,significantly inhibiting endoplasmic reticulum stress and apoptosis caused by excessive iodine intake.

关 键 词：白藜芦醇 甲状腺滤泡细胞 碘过量 内质网应激 细胞凋亡 

分 类 号：R966[医药卫生—药理学] R581[医药卫生—药学] R329.2