衡阳烟区烟草叶斑病病原菌的鉴定  

Identification of the Pathogenic Fungus Causing Tobacco Leaf Spot Disease in Hengyang Region

作　　者：肖志鹏 王灿 袁野 母婷婷 向鹏华 郭维 徐文静 朱校奇 黄艳宁 徐瑞 XIAO Zhipeng;WANG Can;YUAN Ye;MU Tingting;XIANG Penghua;GUO Wei;XU Wenjing;ZHU Xiaoqi;HUANG Yanning;XU Rui(Hunan Tobacco Company Hengyang City Company,Hengyang 421200,China;Hunan Institute of Agricultural Environment and Ecology,Changsha 410125,China;Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南省烟草公司衡阳市公司,湖南衡阳421200 [2]湖南省农业环境生态研究所,湖南长沙410125 [3]湖南农业大学,湖南长沙410128

出　　处：《湖南生态科学学报》2024年第3期98-102,共5页Journal of Hunan Ecological Science

基　　金：烟草叶斑病害早期诊断及生物防治技术研究与应用(HYYC2023KJ34)。

摘　　要：【目的】为明确衡阳烟区新发现的一种烟草未知叶斑病病原菌及其生物学特性。【方法】采用科赫氏法则,对病叶进行分离纯化,形态学观察,ITS、EF-1α、β-tubulin和RPB2序列分析,构建系统发育树以及致病性测定等多种方法分析鉴定。【结果】经观察,气生菌丝边缘整齐,正面呈现深灰色,背面深褐色,分生孢子呈椭圆形[(1.76~2.38)μm×(0.85~1.40)μm(n=50)],ycSQ1菌株ITS区、EF-1α、β-tubulin及RPB2序列与高粱附球菌(GenBank登录号分别为MT341779.1、MF987524.1、OP555979.1及MW899504.1)的序列一致度为99%~100%。构建了基于ITS和β-tubulin序列的系统发育树。ycSQ1与高粱附球菌亲缘关系较近(GenBank登录号分别为MK391054.1和MK391058.1),自展值为88%。因此,经形态学观察、分子生物学鉴定和致病性测定,确定引起衡阳烟草产区叶斑病的病原菌为高粱附球菌(Epicoccum sorghinum)。【结论】本研究首次在衡阳地区发现高粱附球菌作为引起烟草叶斑病的病原菌。这一发现对于有效防控该病害、减少其对烟草生产造成的重大经济损失具有一定的参考价值。【Objective】To clarify the unknown leaf spot pathogen of a newly discovered tobacco leaf disease in Hengyang and its biological characteristics.【Method】Koch s postulates,the isolation,purification,morphological observation,ITS,EF-1α,β-tubulin and RPB2 sequence analysis,construction of phylogenetic tree,as well as pathogenicity determination were employed for the identification and analysis of the diseased leaves.【Result】After observation,the aerial mycelia edge was neat,presenting a deep gray color on the front and a dark brown color on the back.The conidial spores were oval-shaped[(1.76~2.38)μm×(0.85~1.40)μm(n=50)].The ycSQ1 strain showed 99%-100%sequence identity with the sequences of ITS region,EF-1α,β-tubulin,and RPB2 of Epicoccum sorghinum(GenBank accession numbers MT341779.1,MF987524.1,OP555979.1,and MW899504.1).Subsequently,a phylogenetic tree based on the ITS andβ-tubulin sequences was constructed.ycSQ1 and Epicoccum sorghinum exhibited a close relationship(GenBank accession numbers MK391054.1 and MK391058.1),with a bootstrap value of 88%.Therefore,through morphological observation,molecular biology identification,and pathogenicity determination,it was confirmed that the pathogen causing the leaf spot disease in the tobacco production area of Hengyang was Epicoccum sorghinum.【Conclusion】This study marks the first discovery of Epicoccum sorghinum as the pathogen causing tobacco leaf spot disease in Hengyang Region.This discovery holds significant importance in effectively controlling this disease and reducing the major e conomic losses it caused to tobacco production.

关 键 词：烟草 叶斑病 高粱附球菌 分离鉴定 

分 类 号：Q949.32[生物学—植物学] S435.72[农业科学—农业昆虫与害虫防治]