基于MSEP@CTAB的磁固相萃取结合HPLC-UV法测定环境水和饮品中的苏丹红  

Determination of Sudan dyes in environmental water and drinks by MSEP@CTAB-based magnetic solid phase extraction combined with HPLC-UV

作　　者：吴枫 龙瑞宽 周媛媛 王鑫[1] 刘有平[1] 邸欣[1] WU Feng;LONG Ruikuan;ZHOU Yuanyuan;WANG Xin;LIU Youping;DI Xin(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2024年第9期1190-1197,1204,共9页Journal of Shenyang Pharmaceutical University

摘　　要：目的制备一种有机/无机双功能化磁性纳米复合材料MSEP@CTAB,将其作为磁固相萃取的吸附剂并结合HPLC-UV法检测环境水和饮品中的苏丹红残留。方法以Fe_(3)O_(4)作为磁性纳米粒子,选择海泡石(Sepiolite,SEP)为无机材料对其进行修饰,再采用十六烷基三甲基溴化铵(cetyltrimethylammonium bromide,CTAB)进行有机改性,得到有机/无机双功能化磁性纳米复合材料MSEP@CTAB;利用傅里叶变换红外光谱仪、透射电子显微镜、X射线衍射仪、振动样品磁力针和全自动比表面及孔隙度分析仪等对复合材料进行表征;考察并优化磁固相萃取条件。结果在最佳实验条件下,3种苏丹红染料在质量浓度0.5~50 ng·mL^(-1)内线性关系良好(R^(2)≥0.9962),检测限为0.05~0.07 ng·mL^(-1),定量限为0.17~0.23 ng·mL^(-1),日内和日间精密度分别为1.8%~8.8%和2.8%~9.0%。该方法被成功应用于环境水和饮品中3种苏丹红染料的检测,加标回收率为82.5%~108.2%。结论所建立的方法简便、快速、灵敏,为环境水和饮品中痕量苏丹红染料的检测提供了一种可行的策略。Objective An organic/inorganic dual-functionalized magnetic nanocomposite(MSEP@CTAB)was synthesized and used as a sorbent for magnetic solid phase extraction combined with HPLC-UV to detect Sudan dyes residues in drinks and the environment water.Methods Sepiolite(SEP)was used as an inorganic material to modify the Fe3O4 magnetic nanoparticles.After that,organic modification was carried out using cetyltrimethylammonium bromide(CTAB),the organic/inorganic dual-functionalized magnetic nanocomposites MSEP@CTAB was successfully obtained.Fourier transform infrared spectroscopy,transmission electron microscopy,X-ray diffractometer,vibrating sample magnetometer and fully automatic specific surface and porosity analyzer were used to characterize the synthesized materials.The conditions of magnetic solid phase extraction were investigated and optimized.Results Under the optimal experimental conditions,the developed method provided the good linearity(R^(2)≥0.9962)in the range of 0.5-50 ng·mL^(-1)with low limits of detection of 0.05-0.07 ng·mL^(-1).The intra-day and intra-day precision were 1.8%-8.8%and 2.8%-9.0%,respectively.Three Sudan dyes were effectively detected by this approach in drinks and the environment water with spiked recoveries ranging from 82.5%to 108.2%.Conclusion The established method is simple,rapid,sensitive,providing a feasible strategy for the detection of trace amounts of Sudan dyes in environmental water and drinks.

关 键 词：海泡石 十六烷基三甲基溴化铵 磁固相萃取 苏丹红 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]