克林霉素原位富集土壤微生物群落多样性及抗菌活性分析  

Analysis of diversity and antibacterial activity of soil microbial communitiesenriched with clindamycin in soil

作　　者：孔令楠 王磊[1,2] 万传星 KONG Lingnan;WANG Lei;WAN Chuanxing(College of Life Science and Technology,Tarim University,Alar,Xinjiang 843300;State Key Laboratory Breeding Base for The Protection and Utilization of Biological Resources in Tarim Basin Co-funded by Xinjiang Production&Construction Corps and The Ministry of Science&Technology,Alar,Xinjiang 843300)

机构地区：[1]塔里木大学生命科学与技术学院,新疆阿拉尔843300 [2]塔里木盆地生物资源保护利用兵团重点实验室-省部共建国家重点实验室培育基地,新疆阿拉尔843300

出　　处：《塔里木大学学报》2024年第3期21-29,共9页Journal of Tarim University

基　　金：塔里木大学校长基金项目(TDZKKY202201)。

摘　　要：为了探究克林霉素原位富集土壤微生物多样性和优势菌群的抗菌活性,以克林霉素作为选择压进行原位富集,利用Illumina Navaseq高通量测序技术分析土壤微生物群落多样性;通过微生物培养技术结合滤纸片法以及菌饼对峙法,筛选出具有抗菌活性的耐药放线菌。结果表明,随着原位富集土壤中克林霉素添加量的增加,其物种丰度减少。在克林霉素质量浓度为1∶10和1∶100的原位富集土壤微生物中,主要优势菌目为微球菌目(Micrococcales)、弗兰科氏菌目(Frankiales)、棒状杆菌目(Corynebacteriales)、动孢菌目(Kineosporiales)、链霉菌目(Streptomycetales)。可培养水平上,克林霉素原位富集土壤中共分离出放线菌207株,分属于6目6科6属44种,其中链霉菌属(Streptomyces)为优势菌属,并筛选出25株对梨黑斑链格孢菌(Alternaria gaisen)具有较好抑制活性的放线菌。综上所述,添加克林霉素原位富集土壤微生物更有利于分离出对病原真菌产生拮抗的活性菌株,在病原菌生物防治中具有较大的应用前景。In order to investigate the microbial diversity,and the antibacterial activity of the dominant microbes in situ concentration in soil,clindamycin was used as a selecting expressure for the enrichment in situ,and the microbial community diversity in soil was analyzed by using Illumina Navaseq high-throughput sequencing technology and culture-free methods.Meanwhile,resistant actinomycetes were screened out using microbial cultivation techniques,combined with the filter paper method and cake confrontation method.The findings showed that at the culture-free level,with the increased addition of clindamycin in the in soil,the species abundance decreased,while the dominant genera survived.In the concentration of 1∶10 and 1∶100 clindamycin in situ enrichment of soil microorganisms,the main dominant bacteria were Micrococcales,Frankiales,Corynebacteriales,Kineosporiales,and Streptomycetaies.The culturable level,a total of 207 actinomycetes were isolated from the clindamycin-enriched soil in situ,belonging to 44 species,6 genera,6 families,6 orders.Moreover,Streptomyces were the dominant genera,and 25 actinomycetes with excellent activity against Alternaria gaisen were selected.In conclusion,the clindamycin-enriched soil in situ is more conducive to the isolation of activity strains that antagonize pathogenic fungi,has a great prospects in the biological control of pathogens.

关 键 词：细菌多样性 克林霉素 原位富集 耐药放线菌 抗菌活性 

分 类 号：Q939.11[生物学—微生物学]