超高效液相色谱-串联质谱法同时测定出厂水中5种消毒副产物和高氯酸盐  

Simultaneous determination of five disinfection by-products and perchlorate in finished water by UPLC-MS/MS

作　　者：李伟[1] 柯海瑞 李凤 马建敏[1] 张利锋[2] LI Wei;KE Hairui;LI Feng;MA Jianmin;ZHANG Lifeng(Xinxiang Center for Disease Control and Prevention,Xinxiang,Henan 453000,China;Henan Provincial Center for Disease Control and Prevention)

机构地区：[1]新乡市疾病预防控制中心,河南新乡453000 [2]河南省疾病预防控制中心

出　　处：《现代疾病预防控制》2024年第9期652-656,661,共6页MODERN DISEASE CONTROL AND PREVENTION

基　　金：河南省医学科技攻关计划(联合共建)项目(LHGJ20220166)。

摘　　要：目的建立一种高效测定出厂水中二氯乙酸、三氯乙酸、溴酸盐、氯酸盐、亚氯酸盐和高氯酸盐的超高效液相色谱-串联质谱方法。方法样品经0.22μm孔径的亲水滤膜过滤后,在Acclaim Trinity P1色谱柱(100 mm×2.1 mm,3.0μm)上进行分离,采用乙腈-20 mmol/L甲酸铵水溶液梯度洗脱方式,电喷雾负离子模式,多反应监测模式进行质谱分析。通过评价基质效应,采用超纯水配制标准曲线法对6种目标物质进行定量分析。结果6种目标物质在线性范围内均具有良好的线性关系(r≥0.995),检测限为0.068~0.800μg/L,定量限为0.14~2.40μg/L。以自来水为基底进行低、中、高浓度的加标实验,平均回收率为88.6%~105.3%,相对标准偏差范围为1.5%~5.6%。结论该分析方法快速简便、灵敏度高、重现性好,可用于出厂水中微量5种消毒副产物和高氯酸盐的检测。Objective To establish anultra-performance liquid chromatography-tandem mass spectrometry method for the determination of dichloroacetic acid,trichloroacetic acid,bromate,chlorate,chlorite and perchlorate in finished water.Methods The water samples were filtered by 0.22μm hydrophilic membrane,then were separated on the Acclaim Trinity P1 Column(100 mm×2.1 mm,3.0μm)and analyzed using gradient elution of acetonitrile-20 mmol/L ammonium formate,electrospray negative ion mode,and multi-reaction monitoring mode.By comparing the matrix effect,six target substances were quantitatively analyzed by the standard curve method prepared by ultra-pure water.Results The six target substances all showed good linear relationships within the linear range(r≥0.995),with a detection limit of 0.068-0.800μg/L and a quantification limit of 0.14-2.40μg/L.The spike experiments with low,medium and high concentrations were carried out with tap water as the base,and the average recovery rates were from 88.6%-105.3%,and the ranges of relative standard deviation were from 1.5%-5.6%.Conclusions The proposed method is rapid,simple,sensitive and reproducible,and can be used for the detection of the five disinfection byproducts and perchlorate in finished water.

关 键 词：液相色谱-串联质谱法 消毒副产物 高氯酸盐 出厂水 

分 类 号：R123[医药卫生—环境卫生学]