2016—2023年河南省三门峡市A(H1N1)pdm09流感病毒基因特征  

Genetic characterization of influenza A(H1N1)pdm09 virus in Sanmenxia,Henan,2016-2023

作　　者：杨洁[1] 卫成[1] 刘天福 张毅珍[1] 许苗苗 李大攀 赵升[2] YANG Jie;WEI Cheng;LIU Tianfu;ZHANG Yizhen;XU Miaomiao;LI Dapan;ZHAO Sheng(Sanmenxia Center for Disease Control and Prevention,Sanmenxia,Henan 472000,China;Henan Provincial Center for Disease Control and Prevention)

机构地区：[1]三门峡市疾病预防控制中心,河南三门峡472000 [2]河南省疾病预防控制中心

出　　处：《现代疾病预防控制》2024年第9期691-697,共7页MODERN DISEASE CONTROL AND PREVENTION

摘　　要：目的了解河南省三门峡市A(H1N1)pdm09流感病毒血凝素(hemagglutimin,HA)和神经氨酸酶(neuraminidase,NA)基因的进化特征、抗原及受体结合位点变异及其遗传进展。方法选取2016—2023年三门峡市分离的24株A(H1N1)pdm09流感病毒毒株,提取核酸后对流感病毒全基因组进行二代测序,利用生物信息学软件分析HA、NA基因特征,构建系统进化树。结果2016—2023年A(H1N1)pdm09流感病毒毒株的HA、NA核苷酸和氨基酸同源性相对于A/California/07/2009参考株逐年降低,与当年疫苗推荐株相比,同源性仍保持在96%以上,属于6B.1和6B.2分支,且不断进化出6B.1A、6B.1A.5、6B.1A.7等亚分支。与参考株相比,HA1蛋白表现出60个氨基酸突变,在抗原决定簇中有11个突变位点;NA蛋白13个抗原决定簇和药物结合位点共发生45个氨基酸突变。2023年的毒株在HA上发现了8个新的突变位点,包括位于HA抗原决定簇内的A186T、Q189E突变;NA蛋白中出现2个新的突变位点V453M、K469N。在23株毒株中发现了HA1蛋白的Sa抗原决定簇内的162位(162NQSY或162NQT)的额外糖基化位点,其中所有毒株在NA蛋白的42位(NQS)新增1个糖基化位点。结论三门峡市分离的A(H1N1)pdm09流感病毒毒株的HA和NA基因持续发生氨基酸位点变异和糖基化位点增加,这些发现表明加强流感毒株型别及突变监测的必要性。Objective To delineate the evolutionary characteristics,antigenic and receptor binding site variations,and genetic progression of hemagglutinin(HA)and neuraminidase(NA)genes of the influenza virus A(H1N1)pdm09 in Sanmenxia City,Henan Province from 2016 to 2023.Methods A total of 24 A(H1N1)pdm09 influenza virus strains isolated from Sanmenxia City between 2016 and 2023 were selected.Following nucleic acid extraction,the complete influenza virus genome was subjected to second-generation sequencing.Bioinformatics software was applied to analyze the HA and NA gene characteristics and to construct a phylogenetic tree.Results A progressive decrease in HA,NA nucleotide and amino acid homology was observed from 2016 to 2023 relative to the A/California/07/2009 reference strain.Despite this decrease,homology with the annual recommended vaccine strains remained above 96%,with strains classified under the 6B.1 and 6B.2 branches,evolving into sub branches such as 6B.1A,6B.1A.5,6B.1A.7.,HA1 protein exhibited 60 amino acid mutations,with 11 mutation sites in the antigenic determinant cluster,while the NA protein showed 45 amino acid mutations across 13 antigenic determinants and drug binding sites comparing with reference strain.Among 2023 strains,eight new mutation sites were identified on HA,including mutations of A186T and Q189E located within the HA antigen determinant cluster.Two novel mutation sites,V453M and K469N,emerged in the NA protein.An additional glycosylation site at position 162(162NQSY or 162NQT)in the HA1 protein's Sa antigen determinant cluster was found in 23 strains,within and all strains presented anextra glycosylation site at position 42(NQS)in the NA protein.Conclusions The HA and NA genes of the A(H1N1)pdm09 influenza virus isolated in Sanmenxia City have undergone continuous amino acid mutations and an increase in glycosylation sites.These findings highlight the necessity to enhance the surveillance of influenza virus types and mutations.

关 键 词：流感病毒 血凝素 神经氨酸酶 抗原决定簇 变异位点 进化树 

分 类 号：R373.13[医药卫生—病原生物学]