Study on the effect of Shixiang plaster on the expression of CD31,serum FN,and VEGF in a rat model with chronic wounds  

作　　者：Ji Fei Ling-Li Wang Man Liu Peng Liu Jing-Hua Ruan Kai-Wei Zhang 

机构地区：[1]College of Chinese Orthopedics and Traumatology,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China [2]Pharmaceutic Preparation Centre,The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China

出　　处：《Traditional Medicine Research》2024年第12期31-40,共10页TMR传统医学研究

基　　金：supported by the Guizhou Provincial Traditional Chinese Medicine Administration Traditional Chinese Medicine and Ethnic Medicine Scientific Research Project(Project number:QZYY-2023-013).

摘　　要：Background:Chronic wounds pose a significant surgical challenge,often requiring traditional treatments with limited efficacy.This study explores the promising impact of Shixiang plaster,a classic Chinese ointment,on wound healing.We investigated the cluster of differentiation 31(CD31)expression,serum fibronectin(FN),and vascular endothelial growth factor(VEGF)levels in SPF rats with induced wounds to elucidate the mechanism behind Shixiang plaster’s effectiveness.We investigated the effect and explored the role of Shixiang plaster on the expression of CD31,serum FN,and VEGF in chronic wounds.Methods:The study involved 36 SPF rats divided into model,rb-bFGF,and Shixiang plaster groups.Penicillin was injected into the rats before modelling for 3 days to prevent infection.The skin was excised 2 cm below the horizontal line of the inferior border of the shoulder bone in the middle of the rat column up to the deep fascial layer and inoculated with a certain concentration of Staphylococcus aureus;the wound was covered aseptically for 3 days.The trauma area of the rats was observed at 3,7,and 14 days,respectively.Histopathology was observed using haematoxylin eosin and Masson staining.CD31 expression was detected using immunohistochemistry staining.FN and VEGF expression was detected using serum ELISA.Statistical analyses were carried out by the method of SPSS.Results:Regarding wound morphology,at 3 days,the recovery area of the Shixiang plaster group was larger than that of the other two groups,at 7 days,the wound healing rate of the Shixiang plaster group was significantly higher,and at 14 days,the wounds of the Shixiang plaster group had been mostly healed,with a healing rate of 98.3%.Haematoxylin eosin staining revealed a large amount of granulation tissue at 3 days in the Shixiang plaster group,and the epidermal scales disappeared at 14 days,with thinner epidermal thickness at 1 lesion and a large reduction in inflammatory cell infiltration.Masson staining showed that at 3,7,and 14 days,blue staining was the most

关 键 词：Shixiang plaster chronic wounds CD31 FN VEGF 

分 类 号：R285.5[医药卫生—中药学]