江西省部分地区鸽源大肠杆菌分离鉴定及耐药性分析  

Isolation,identification and antimicrobial drug resistance analyses of Escherichia coli from pigeons in some areas of Jiangxi Province

作　　者：曾庆华 杨宇玲 雷组建 彭秀兰 胡颖 聂江江 胡煜 王珂 邬向东[1] 吴欢生[1] ZENG Qinghua;YANG Yuling;LEI Zujian;PENG Xiulan;HU Ying;NIE Jiangjiang;HU Yu;WANG Ke;WU Xiangdong;WU Huansheng(School of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China;School of Forestry,Jiangxi Agricultural University,Nanchang 330045,China;Bureau of Animal Husbandry and Veterinary of Yugan,Shangrao,Jiangxi 335100,China;Service Center for Agricultural and Rural Industrial Development of Anfu,Ji’an,Jiangxi 343200,China)

机构地区：[1]江西农业大学动物科学技术学院/江西省畜禽疾病诊断与防治重点实验室,江西南昌330035 [2]江西农业大学林学院,江西南昌330035 [3]江西省余干县畜牧兽医局,江西上饶335100 [4]安福县农业农村产业发展服务中心,江西吉安343200

出　　处：《生物灾害科学》2024年第3期458-466,共9页Biological Disaster Science

基　　金：江西省林业局樟树研究专项(2020CXZX07-0405)。

摘　　要：【目的】为研究鸽源大肠杆菌对常见抗菌药物的耐药情况。【方法】从江西省高安市五家肉鸽养殖场采集125份明显腹泻鸽的肛拭子,将样品处理后采用平板划线法进行细菌分离,多次纯化后进行菌种鉴定和20种常见抗生素的药敏试验,统计数据后进行药物敏感性分析、多重耐药分析和耐药谱分析。【结果】共分离得到102株大肠杆菌,阳性率为84.30%;对20种常见抗菌药物进行药物敏感性检测,其中对四环素(97.06%)耐药率最高,其次是多西环素(95.10%)、氨苄西林(92.16%)、阿莫西林(90.20%)、链霉素(86.27%)、复方新诺明(82.35%)、氯霉素(73.53%)、氟苯尼考(72.55%)、萘啶酸(71.57%)、头孢噻肟(63.73%),其余均在60%以下,而对庆大霉素(44.11%)、头孢唑林(52.94%)、头孢曲松(59.80%)、阿米卡星(48.04%)、氨曲南(52.94%)表现为高敏;分离大肠杆菌呈现不同程度的多重耐药,均在4重以上,以10~15重耐药为主,多重耐药率达到100%;共产生92种耐药谱组合。【结论】该地区鸽大肠杆菌病发病率较高,且其对常见抗菌药物的耐药问题已相当严重。[Objective]This study was conducted to study the resistance of pigeon Escherichia coli to common antibacterial drugs.[Method]125 anal swabs from pigeons with diarrhea symptoms were collected from five meat pigeon farms in Gao’an City,Jiangxi Province.After the samples were treated,the bacteria were isolated by plate streaking method.After multiple purification,the strains were identified and the drug sensitivity test for 20 common antibiotics was carried out.After data statistics,drug sensitivity analysis,multi-drug resistance analysis and drug resistance spectrum analysis were carried out.[Result]The results showed that 102 strains of Escherichia coli were isolated,and the positive rate was 84.30%.And the drug resistance rate to tetracycline(97.06%)was the highest,followed by that of doxycycline(95.10%),ampicillin(92.16%),amoxicillin(90.20%),streptomycin(86.27%),cotrimoxazole(82.35%),chloramphenicol(73.53%),florfenicol(72.55%),nalidixic acid(71.57%),cefotaxime(63.73%),and that of others were below 60%.The isolated Escherichia coli were highly sensitive to gentamicin(44.11%),cefazolin(52.94%),ceftriaxone(59.80%),amikacin(48.04%)and aztreonam(52.94%).The isolated Escherichia coli showed different degrees of multi-drug resistance,all of which were more than 4 times,mainly 10-15 times,and the rate of multi-drug resistance reached 100%.Especially,92 drug resistance spectrum combinations were produced.[Conclusion]The incidence of pigeon colibacillosis in this area is high,and its resistance to common antibiotics has been quite serious.

关 键 词：鸽源大肠杆菌 分离鉴定 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]