水稻云恢1973恢复基因关联分子标记筛选及应用  

Screening of Molecular Markers Associated with Restoring Gene in Yunhui 1973 and Their Application

作　　者：杨丹丹 吕莹 董阳均 徐雨然[1,3,4] 奎丽梅 涂建[1,3,4] 张笑 邓伟[1,3,4] 李小林 YANG Dandan;LYU Ying;DONG Yangjun;XU Yuran;KUI Limei;TU Jian;ZHANG Xiao;DENG Wei;LI Xiaolin(Food Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Weixin County Seed Administration Station,Weixin Yunnan 657900,China;Yunan Main Crop Seed Processing Engineering Technology Research Center,Kunming 650205,China;Yunnan Key Laboratory of Rice Genetic Improvemen,Kunming 650205,China;Shuifu County Seed Administration Station,Shuifu Yunnan 657800,China)

机构地区：[1]云南省农业科学院粮食作物研究所,昆明650205 [2]威信县种子管理站,云南威信657900 [3]云南省主要农作物种子加工工程技术研究中心,昆明650205 [4]云南省稻作遗传改良重点实验室,昆明650205 [5]水富县种子管理站,云南水富657800

出　　处：《西南大学学报（自然科学版）》2024年第9期55-64,共10页Journal of Southwest University(Natural Science Edition)

基　　金：现代农业产业技术体系建设专项(CARS-01-102);粮食安全种业支撑专项(530000210000000013809);云南省科技人才与平台计划项目(202105AE160009);云南省重大科技专项计划项目(202202AE090003)。

摘　　要：恢复系作为三系杂交稻关键亲本之一,在杂种后代的性状方面发挥着主要的调控作用,但目前生产上用的水稻恢复系同质化严重,创制遗传背景丰富的新恢复系显得尤为重要.选用WA型强恢复系—云恢1973和WA型不育系广8A构建F_(2)群体,筛选与云恢1973恢复基因紧密连锁的标记,利用筛选到的标记对云恢1973与云恢290构建的重组自交系材料进行恢复基因的快速扫描;筛选出含有云恢1973恢复基因的重组自交系材料进行后代育性及农艺性状鉴定,筛选出新的具有丰富遗传背景的恢复系,为杂交水稻的可持续发展奠定物质基础.结果表明:在2,10,11号染色体上筛选到12个与育性恢复紧密连锁的分子标记,综合12个分子标记结果,于300株云恢290/云恢1973重组自交系中筛选到72份含恢复基因的新恢复系材料;在峨山和水富试验点对72份新恢复系材料进行鉴定,结果显示72份新恢复系花粉育性均表现为正常,单株有效穗在10穗以上的材料有11份,每穗粒数在250粒以上的材料有8份,千粒质量在30.53 g以上的材料有8份,结实率在80%以上的材料有8份,单株产量高于对照的材料有19份,并有6份低直链淀粉含量材料、29份高直链淀粉含量材料.Restorer line,as one of the key parents of three-line hybrid rice,plays a major regulatory role in the traits of hybrid offspring.However,the current rice restorer lines used in production are highly homogenized,therefore,it particularly important to create new restorer lines with rich genetic backgrounds.WA type strong restoring line Yunhui 1973 and male sterile line Guang 8A were selected to construct an F2 population for screening the markers closely linked to the restoring genes of Yunhui 1973,and using the selected markers for rapidly scanning for the restoring genes from the recombinant inbred line materials constructed with Yunhui 1973 and Yunhui 290.The recombinant inbred lines containing the Yunhui 1973 restoration gene were screened to identify the fertility and agronomic traits of their offspring,and new restoration lines with rich genetic background were screened to lay a material foundation for the sustainable development of Hybrid rice.The results indicate that 12 molecular markers closely linked to fertility restoration were selected on chromosomes 2,10,and 11,and 72 new restoring line materials containing restoring genes were selected from 300 Yunhui 290/Yunhui 1973 recombinant inbred lines.72 new restoring line materials were evaluated in Eshan and Shuifu experimental sites.The results showed that the pollen fertility of the 72 new restoring line materials was normal.There were 11 materials with more than 10 effective panicles per plant,8 materials with more than 250 grains per panicle,8 materials with more than 30.53 g of thousand grain weight,8 materials with over 80% of seed setting rate,19 materials with higher yield per plant than the control,6 materials with low amylose content and 29 materials with high amylose content.

关 键 词：水稻 恢复系 恢复基因 分子标记辅助选择 

分 类 号：S511[农业科学—作物学]