白头翁汤对湿热泄泻大鼠回肠黏膜屏障功能的影响  

Effects of Pulsatilla Decoction on the Barrier Function of Ileal Mucosa in Rats with Damp-Heat Diarrhoea

作　　者：张丽芳 姜盛铭 马琪 杜红旭 毕师诚 曹立亭[1] ZHANG Lifang;JIANG Shengming;MA Qi;DU Hongxu;BI Shicheng;CAO Liting(College of Veterinary Medicine,Southwest University,Rongchang Chongqing 402460,China)

机构地区：[1]西南大学动物医学院,重庆荣昌402460

出　　处：《西南大学学报（自然科学版）》2024年第9期85-96,共12页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(32202849);重庆市自然科学基金项目(CSTB2022NSCQ-MSX0470);国家生猪技术创新中心先导科技项目(NCTIP-XD/C17)。

摘　　要：为探究白头翁汤(Pulsatilla Decoction,PD)对湿热泄泻(Damp-heat Diarrhea,DHD)大鼠回肠黏膜屏障功能的影响,选用48只雄性SD大鼠随机分为空白组(NC)、模型组(Model)、自愈组(SH)、白头翁汤高剂量组(PD-H)、白头翁汤中剂量组(PD-M)、白头翁汤低剂量组(PD-L).以“高糖高脂+高热高湿+肠毒性大肠杆菌”复杂因素诱导大鼠DHD模型,模型建立成功后,PD组大鼠分别以0.752 g/mL(PD-H),0.376 g/mL(PD-M),0.188 g/mL(PD-L)进行灌胃治疗,SH组和NC组大鼠等量生理盐水灌胃,每日1次,连续5 d.试验期间,记录大鼠体质量及采食量变化,试验结束后,采集血液进行血常规指标检测并测定血浆炎症细胞因子;采集心、肝、脾、肺、肾称质量并计算脏器指数;采集回肠组织进行病理组织学观察并检测炎症反应、黏膜屏障及色氨酸通路相关基因mRNA表达水平.结果显示,与NC组比较,Model组大鼠采食量、体质量变化率降低,心、肝、脾、肺、肾脏器指数均显著上升(p<0.05),淋巴细胞数(Lymph)、单核细胞数(Mon)、平均红细胞体积(MCV)显著降低(p<0.05),中性粒细胞绝对值(Gran)、淋巴细胞百分比(Lymph%)、红细胞数(RBC)、血小板数(PLT)显著升高(p<0.05),血浆IL-1β,IL-6水平显著上升(p<0.05),回肠绒毛排列杂乱、固有层明显增厚、部分隐窝结构有消失、杯状细胞数量减少、微绒毛形成的纹状缘弥漫性缺失、乳糜管内有红细胞浸润、肠绒毛高度/隐窝深度(V/C)显著降低(p<0.05),回肠IL-1β,IL-6和IFN-γ,TNF-α,IL-17 mRNA水平显著升高(p<0.05),IL-4,ZO-1 mRNA水平显著降低(p<0.05),TPH1,KMO,IDO-1,MUC1,MUC2 mRNA水平显著升高(p<0.05);与Model组比较,PD-H组大鼠Lymph,MCV显著升高(p<0.05)、 PD-M组大鼠Lymph,Mon,PLT均显著降低(p<0.05),PD-L组大鼠HGB显著降低(p<0.05)、 MCV显著升高(p<0.05),PD-M大鼠肝脏指数显著降低(p<0.05),PD-H组、 PD-M组、 PD-L组大鼠脾脏指数均显著降低(p<0.05),PD-H组、 PD-L组大鼠肺脏、�To investigate the effect of Pulsatilla Decoction(PD) on the mucosal barrier function of ileum of rats with Damp-heat Diarrhea(DHD).Forty-eight male SD rats were selected and randomly divided into blank group(NC),model group(Model),self-healing group(SH),pulsatilla high dose group(PD-H),pulsatilla medium dose group(PD-M),and pulsatilla low dose group(PD-L).The rat DHD model was induced by the complex factors of “high sugar,high fat,high heat,high humidity and enterotoxigenic E.coli”.After the model was successfully established,rats in the PD group were treated with 0.752 g/mL(PD-H),0.376 g/mL(PD-M),and 0.188 g/mL(PD-L) by gavage,respectively,while the rats in the SH and NC groups were garaged with an equal amount of saline once a day for 5 d.During the test period,the changes of body weight and food intake of the rats were recorded;at the end of the test period,blood was collected for the detection of hematological indexes and determination of plasma inflammatory cytokine content.Heart,liver,spleen,lungs and kidneys were collected and weighed to calculate the organ indexes.Ileal tissues were collected for pathological and histological observation and detection of inflammatory reactions,and expression levels of the genes related to mucosal barrier and tryptophan pathway.The results showed that compared with the NC group,rats in the Model group had lower feed intake,lower growth rate of body mass,significantly higher heart,liver,spleen,lung and kidney organ indexes(p<0.05),significantly lower Lymph,Mon and MCV(p<0.05),significantly higher Gran,Lymph%,RBC and PLT(p<0.05),significantly higher serum IL-1β and IL-6(p<0.05),and disorganized ileal villi with significant thickening of the lamina propria(p<0.05).Some of the crypt structures were disappeared,the number of cup cells was decreased,the striatal rim formed by microvilli was diffusely missing.There was erythrocyte infiltration in the celiac duct,the height of the intestinal villi/crypt depth was significantly reduced(p<0.05).The mRNA levels of ileal IL-1β,

关 键 词：湿热泄泻 白头翁汤 回肠组织 黏膜屏障 

分 类 号：S853.6[农业科学—临床兽医学]