安徽省2018—2021年128株肠炎沙门菌耐药及分子分型分析  

Analysis of drug resistance and molecular typing of 128 Salmonella enteritis strains in Anhui Province from 2018 to 2021

作　　者：马泰 张竹慧[1] 撒楠[1] 王昕昕 栗薇薇[1] 孙永[1] MA Tai;ZHANG Zhuhui;SA Nan;WANC Xinxin;LI Weiwei;SUN Yong(Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,Anhui,China)

机构地区：[1]安徽省疾病预防控制中心,安徽合肥230601

出　　处：《安徽预防医学杂志》2024年第4期301-304,共4页Anhui Journal of Preventive Medicine

基　　金：国家科技重大专项(2018ZX10713003-002-005)。

摘　　要：目的了解安徽省肠炎沙门菌耐药谱及其分子分型特征。方法对安徽省6个市2018—2021年分离培养的128株肠炎沙门菌进行药敏实验,采用脉冲场凝胶电泳(PFGE)进行分子分型,运用Bio Numerics 6.6软件对PFGE图谱进行聚类分析。结果128株肠炎沙门菌对14种抗生素产生不同程度耐药,耐药率最高的是萘啶酸(98.43%,126/128),其次是氨苄西林(87.50%,112/128)、氨苄西林/舒巴坦(69.53%,89/128)。128株肠炎沙门菌基因组切分为35种带型,各种带型间相似度为67.10%~100.00%。结论安徽省6个市肠炎沙门菌耐药率较高,PFGE带型相似度较高,应加强传染源调查,规范抗生素使用。Objective To understand the drug resistance spectrum and molecular typing characteristics of Salmonella enteritis in Anhui Province.Methods 128 strains of Salmonella enteritidis isolated and cultured in 6 cities of Anhui Province from 2018 to 2021 were tested for drug sensitivity.The molecular typing was performed by pulsed field gel electrophoresis(PFGE),and the cluster analysis of PFGE patterns was performed by Bio Numerics 6.6 software.Results The 128 strains of Salmonella enterica were resistant to 14 antibiotics to varying degrees,and the highest resistance rate was nalidixic acid(98.43%,126/128),followed by ampicillin(87.50%,112/128),and ampicillin/sulbactam(69.53%,89/128).The genomes of the 128 strains of Salmonella enterica were sectioned into 35 banding types,and the similarity among the various banding types ranged from 67.10%to 100.00%.Conclusion The drug resistance rate of Salmonella enteritidis was high in six cities in Anhui Province,and the similarity of PFGE banding was high,so the investigation of infectious sources should be strengthened and the use of antibiotics should be standardized.

关 键 词：沙门菌 耐药性 脉冲场凝胶电泳 分子分型 

分 类 号：R373.2[医药卫生—病原生物学]