远缘杂种牡丹分生结节诱导及不定芽分化  

Meristematic Nodule Induction and Adventitious Shoot Differentiation in Distant Hybrid Tree Peony

作　　者：谢钰 刘婉婷 崔珺 成仿云[1] 钟原[1] XIE Yu;LIU Wanting;CUI Jun;CHENG Fangyun;ZHONG Yuan(School of Landscape Architecture,Peony International Institute,State Key Laboratory of Efficient Production of Forest Resources,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,National Engineering Research Center for Floriculture,Beijing For‐estry University,Beijing 100083)

机构地区：[1]北京林业大学园林学院,牡丹国际研究院,林木资源高效生产全国重点实验室,花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,北京100083

出　　处：《植物研究》2024年第5期702-710,共9页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31971701)。

摘　　要：该研究以亚组间和组间远缘杂种牡丹的叶柄为外植体,探索一种操作简便、适用于多个品种的分生结节诱导方法,并在部分品种中研究分生结节分化不定芽的条件。结果表明:将11个品种的叶柄薄层接入含有0.5 mg·L^(-1)萘乙酸(NAA)+1.0 mg·L^(-1)氯吡脲(CPPU)+0.5(或0)mg·L^(-1)噻苯隆(TDZ)的SH培养基中,在黑暗条件下培养60~100 d,可以在10个品种中一步诱导出分生结节,诱导率达到52.08%~83.33%;一步法诱导的分生结节是通过愈伤组织间接产生。将分生结节接入含有0.5(或1)mg·L^(-1) CPPU+0.5 mg·L^(-1) TDZ的改良WPM培养基中,在光照、每15 d继代一次的条件下,可以诱导3个品种的不定芽分化,分化率为6.67%~33.33%;较低浓度的CPPU和TDZ共同使用对于不定芽分化有促进作用,而单独使用高浓度的CPPU不利于不定芽分化。该研究筛选出了一步法诱导分生结节的通用培养基,并在部分品种中实现了不定芽分化,为建立远缘杂种牡丹植株再生体系提供了重要参考。To explore a simple and suitable method for inducing meristematic nodules in multiple cultivars,the petioles of inter-subsectional and inter-sectional distant hybrid tree peony were used as explants,and the conditions for the differentiation of meristematic nodules into adventitious shoot in some cultivars were also identified.The results showed that meristematic nodules were induced by one step in 10 cultivars with 52.08%-83.33%induction rates,the thin layers of petioles of 11 cultivars were continuously cultured on SH medium containing 0.5 mg·L^(-1) NAA+1.0 mg·L^(-1) CPPU+0.5(or 0)mg·L^(-1) TDZ for 60-100 d in the dark,and meristematic nodules induced by one step were generated indirectly from the callus.Adventitious shoot differentiation rate of three cultivars was 6.67%-33.33%when meristem nodules were added to the modified WPM medium containing 0.5(or 1)mg·L^(-1) CPPU+0.5 mg·L^(-1) TDZ under light condition and sub-cultured every 15 days.Lower concentrations of CPPU and TDZ together promoted adventitious shoot differentiation,but high concentrations of CPPU alone were not conductive to adventitious shoot differentiation.The general medium for one-step induction of meristematic nodules was screened,and adventitious shoot differentiation was achieved in some cultivars,which provided an important reference for the establishment of plant regeneration system for distant hybrid tree peonies.

关 键 词：亚组间杂种牡丹 组间杂种 分生结节 不定芽 

分 类 号：S685.11[农业科学—观赏园艺]