植物IPP基因的生物信息学分析  

The Bioinformatic Analysis on the Plant Isopentenyl Pyrophosphate Isomerase

作　　者：喻心怡 冀慧玥 路萍萍 周嘉裕[1] 廖海[1] YU Xinyi;JI Huiyue;LU Pingping;ZHOU Jiayu;LIAO Hai(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031)

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《植物研究》2024年第5期774-782,共9页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(32270410)。

摘　　要：异戊烯基焦磷酸异构酶(IPP)催化异戊烯焦磷酸与二甲基丙烯基焦磷酸(DMAPP)的可逆异构化反应,对下游萜类产物的合成发挥重要作用。该研究利用生物信息学方法,从稻(Oryza sativa)与拟南芥(Arabidopsis thaliana)等单、双子叶植物基因组中鉴定获得31条IPP基因。系统进化树将IPP分为3簇,其中单子叶植物单独形成一簇,同簇的IPP成员具有相似的结构域,可能执行相似的生物学功能;以稻与拟南芥IPP基因为代表,顺式调控元件分析显示它们含有干旱等响应元件;定量PCR结果显示,稻与拟南芥IPP转录水平的表达模式较为多样化。研究结果为系统认识IPP基因的生物学作用及可能的应用研究提供了理论依据。Isopentenyl pyrophosphate isomerase(IPP)is responsible for the reversible isomerization of isopentenyl pyrophosphate and dimethylallylpyrophosphate(DMAPP),playing an important role in the biosynthesis of downstream terpenoid products.In this paper,31 IPP genes were identified from genomes of various mono-and dicotyledonous plants such as Oryza sativa and Arabidopsis thaliana by bioinformatics method.Phylogenetic analysis indicated that these IPPs were divided into three clades,in which those from monocotyledon plants formed a monophyletic clade.IPPs within the same clade shared similar domains and might have similar biological functions.The analysis on cis-regulatory elements of O.sativa and A.thaliana IPP genes exhibited multiple response elements such as drought response elements.Finally,quantitative PCR results showed that the expression patterns of IPP transcription levels in O.sativa and A.thaliana were diversified.These above results provided theoretical basis for systematic understanding and further application of IPPs.

关 键 词：异戊烯基焦磷酸异构酶 生物信息学 干旱胁迫 脱落酸 表达模式 

分 类 号：Q945.78[生物学—植物学]