杜仲叶黄酮纯化及其抗炎活性研究  

Purification of the flavonoids from the flavoid extract of the Eucommia leaves and its antibacterial activity

作　　者：杨兴变[1] 陈震 陈伟 苏娇娇 何珺[1] 欧琴 YANG Xingbian;CHEN Zhen;CHEN Wei;SU Jiaojiao;HE Jun;OU Qin(Biochemical Engineering Center of Guizhou University,Guiyang 550025,China;Guizhou Beiyinte Biotechnology Co.,Ltd.,Guiyang 550025,China;Wuhan Wuyao Pharmaceutical Co.,Ltd.,Wuhan 430000,China)

机构地区：[1]贵州大学生化工程中心,贵州贵阳550025 [2]贵州贝因特生物技术有限公司,贵州贵阳550025 [3]武汉武药制药有限公司,湖北武汉430000

出　　处：《应用化工》2024年第8期1823-1826,共4页Applied Chemical Industry

基　　金：贵州省教育厅特色领域项目(黔教合KY字[2020]055)。

摘　　要：对杜仲叶总黄酮提取物及其4种单体进行纯化,并对其抗炎作用进行初步研究。含量65.19%总黄酮杜仲叶提取物通过液-质联用确定其活性组分,经95%乙醇萃取,萃取料液比为1 g∶10 mL,萃取次数为1次,得到80.35%总黄酮含量杜仲叶提取物,通过半制备液相色谱分离,得到槲皮素-3-O-桑布双糖苷、异槲皮苷、芦丁、紫云英苷4种单体黄酮化合物;经测定,4种杜仲叶单体黄酮化合物和杜仲叶18.14%总黄酮提取物及其78.78%总黄酮提取物均有一氧化氮致炎抗炎活性,其中含量为78.78%的总黄酮提取物抗炎活性最好,而单体化合物的一氧化氮生成抑制率为紫云英苷>芦丁>槲皮素-3-O-桑布双糖苷>异槲皮苷。The total flavonoids and four monomer compounds were purified from the Eucommia leaves flavoid extract,anti-inflammatory effects were preliminarily studied.The total flavonoid extract from Eucommia ulmoides leaves with a content of 65.19%,liquid quality analysis of its total flavonoids extract,solid-liquid extraction was used to further purification.The results showed that 95%ethanol extraction,extraction material-to-liquid ratio of 1 g∶10 mL,and extraction frequency of 1 time were used,the extract with a total flavonoid content of Eucommia leaf was up to 80.35%;semi-prepared liquid phase was separated the eucommia flavonoid monomer compound;the anti-inflammatory activity was evaluated by measuring the inhibition effect of NO generation.Four monomer compounds were obtained by semi-prepared liquid phase separation,the four monomer compounds were quercetin-3-O-Sambunisglycoside,isoquercetin,rutin and viola vaeconin;they all had NO inhibition effect of NO generation,and the total flavonoid extract with 78.78%content was best,while monomer compound was viola vaeconin>rutin>quercetin-3-O-Sambunisglycoside>isoquercetin.

关 键 词：杜仲叶黄酮提取物 黄酮纯化 抗炎活性测试 

分 类 号：R284.2[医药卫生—中药学]