适配骨组织工程的蚕丝增强聚己内酯复合材料理化及生物特征研究  

Physical,chemical,and biological property of silk reinforced polycaprolactone composites for bone tissue engineering

作　　者：田文晗 何观平 刘玉增[2] 管娟 TIAN Wenhan;HE Guanping;LIU Yuzeng;GUAN Juan(School of Materials Science and Engineering,Beihang University,Beijing,100083,P.R.China;Department of Orthopedics,Beijing Chaoyang Hospital,Capital Medical University,Beijing,100020,P.R.China;Beijing Advanced Innovation Center for Biomedical Engineering,Beijing,102402,P.R.China)

机构地区：[1]北京航空航天大学材料科学与工程学院,北京100083 [2]首都医科大学附属北京朝阳医院骨科,北京100020 [3]北京市生物医学工程高精尖创新中心,北京102402

出　　处：《中国修复重建外科杂志》2024年第9期1123-1129,共7页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：北京航空航天大学青年科学家团队项目(KG16218401)。

摘　　要：目的报道一种与骨组织力学适配的可降解骨植入材料,为临床骨再生与修复提供新材料。方法通过叠层组装与热压复合技术,将天然蚕丝织物与医用聚己内酯(polycaprolactone,PCL)物理混合,制备含20%、40%、60%蚕丝的蚕丝增强PCL复合材料(silk reinforced PCL composite,SPC)。大体观察形态并采用扫描电镜观察微观结构,以压缩试验检测其压缩力学性能,表面接触角测试检测材料表面润湿性,于PBS缓冲液中浸泡180 d后观测材料降解情况,采用细胞计数试剂盒8法检测MC3T3-E1细胞在20%-/40%-SPC上的增殖情况。取6只SD大鼠,背部皮下分别植入PCL和20%-SPC两种材料,采用Masson染色分析其180 d内体内降解特征和血管化效果。结果3种SPC断面的孔隙缺陷均较少。在20%~60%区间内,随着蚕丝含量增加、PCL含量越来越少,蚕丝织物层间距变小,纤维几乎布满整个断面;SPC的压缩模量和压缩强度呈增大趋势,60%-SPC压缩模量略低于40%-SPC,各材料间压缩模量和压缩强度比较差异均有统计学意义(P<0.05)。体外模拟体液降解实验显示,3种SPC降解180 d的质量损失均在5%以内,其中60%-SPC的质量损失最大,各材料间质量损失比较差异均有统计学意义(P<0.05)。随蚕丝含量增加,各材料静态水接触角逐渐减小,且均可促进MC3T3-E1细胞增殖。大鼠皮下降解实验示,20%-SPC植入后30 d开始降解,180 d材料降解和血管化显著,与PCL形成鲜明对比。结论SPC具有与骨组织适配的力学特性和亲水特性,在体外模拟体液环境中长期保持力学强度,在体内通过机体免疫调控机制,实现材料降解和组织再生及血管化动态同步,有望为临床骨修复提供一种新型植入材料。Objective To develop a biodegradable implantable bone material with compatible mechanics with the bone tissue,providing a new biomaterial for clinical bone repair and regeneration.Methods Silk reinforced polycaprolactone composites(SPC)containing 20%,40%,and 60%silk were prepared by layer-by-layer assembly and hotpressing technology.Macroscopic morphology was observed and microstructure were observed by scanning electron microscopy,compressive mechanical properties were detected by compression test,surface wettability was detected by surface contact angle test,degradation of materials was observed after soaking in PBS for 180 days,and proliferation of MC3T3-E1 cells was detected by cell counting kit 8 assay.Six Sprague Dawley rats were subcutaneously implanted with polycaprolactone(PCL)and 20%-SPC,respectively.Masson staining was used to analyze the in vivo degradation behavior and vascularization effect within 180 days.Results The pore defects of the three SPC sections were relatively few.In the range of 20%to 60%,as the silk content increased and the PCL content decreased,the interlayer spacing of silk fabric decreased,and the fibers almost covered the entire cross-section.The compressive modulus and compressive strength of SPC showed an increasing trend,and the compressive modulus of 60%-SPC was slightly lower than that of 40%-SPC.There were significant differences in compressive modulus and compressive strength between the materials(P<0.05).In vitro simulated fluid degradation experiments showed that the mass loss of the three types of SPC after 180 days of degradation was within 5%,with the highest mass loss observed in 60%-SPC.The differences in mass loss between the materials were significant(P<0.05).As the silk content increased,the static water contact angle of each material gradually decreased,and all could promote the proliferation of MC3T3-E1 cells.The subcutaneous degradation experiment in rats showed that 20%-SPC began to degrade at 30 days after implantation,and material degradation and vasculariza

关 键 词：骨组织工程 可降解生物材料 蚕丝纤维 聚己内酯 纤维增强复合材料 理化及生物特征 

分 类 号：R318.08[医药卫生—生物医学工程] R68[医药卫生—基础医学]