模拟氮沉降对猫儿山亚热带常绿阔叶林土壤细菌多样性和群落结构的影响  

Effects of Simulated Nitrogen Deposition on Diversities and Community Structures of Soil Bacteria in Subtropical Evergreen Broad-leaved Forests of Mao'er Mountain

作　　者：蒋行健 欧芷阳[1,2,3] 谭长强 何琴飞[1,2,3] 郑威 谭一波[1,2,3] 何峰 沈浩[1,2,3] Jiang Xingjian;Ou Zhiyang;Tan Zhangqiang;He Qinfei;Zheng Wei;Tan Yibo;He Feng;Shen Hao(Guangxi Forestry Research Institute,Nanning,Guangxi 530002,China;Guangxi Forestry Laboratory,Nanning,Guangxi 530002,China;Guangxi Lijiangyuan Forest Ecosystem Research Station,Xing'an Guilin Lijangyuan Forest Ecosystem Observation and Research Station of Guangxi,Guilin,Guangxi 541316,China)

机构地区：[1]广西壮族自治区林业科学研究院,广西南宁530002 [2]广西林业实验室,广西南宁530002 [3]广西漓江源森林生态系统国家定位观测研究站,桂林兴安漓江源森林生态系统广西野外科学观测研究站,广西桂林541316

出　　处：《广西林业科学》2024年第4期430-437,共8页Guangxi Forestry Science

基　　金：广西林业科技推广示范项目(2023GXLK13);广西林科院基本科研业务费项目(林科201815号);广西林业科技项目(桂林科研[2015]第34号)。

摘　　要：为了解大气氮沉降下猫儿山土壤细菌群落的变化规律,以猫儿山亚热带常绿阔叶林为对象,设置4种不同施氮处理[0(CK)、50(Ⅱ)、100(Ⅲ)和150(Ⅳ)g·m^(-2)·a^(-1)],采用高通量测序技术研究不同施氮量对土壤细菌多样性和群落结构的影响。结果表明,处理Ⅲ的特有土壤细菌OTU数量最多。随施氮量增加,辛普森指数降低,处理Ⅱ的辛普森指数最高;随施氮量增加,香农和Chao 1指数均呈先升后降的趋势,处理Ⅲ的香农和Chao 1指数均最高。土壤细菌群落以酸杆菌门(Acidobacteria)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)、疣微菌门(Verrucomicrobia)和放线菌门(Actinobacteria)为主。NMDS和聚类分析表明,不同施氮处理未造成细菌群落结构明显分离。LDA分析表明,处理Ⅲ的差异性物种数量最多,绿弯菌门可作为氮添加后的指示性物种。100 g·m^(-2)·a^(-1)施氮量最有利于土壤细菌生长,150 g·m^(-2)·a^(-1)施氮量对土壤细菌生长有一定抑制作用。In order to understand change laws of soil bacterial communities in Mao'er mountain under atmospheric nitrogen deposition,taking subtropical evergreen broad-leaved forests of Mao'er mountain as research objects,four different nitrogen application treatments[0(CK)、50(Ⅱ)、100(Ⅲ)and 150(Ⅳ)g·m^(-2)·a^(-1)]were set up.Effects of different nitrogen applications on diversity and community structure of soil bacteria were studied by high-throughput sequencing technology.Results showed that treatmentⅢhad the highest number of unique soil bacterial OTUs.Simpson index decreased with increase of nitrogen application.TreatmentⅡhad the highest Simpson index.Shannon and Chao 1 indexes showed trends of increase firstly and then decrease with increase of nitrogen application.TreatmentⅢhad the highest Shannon and Chao 1 indexes.Soil bacterial communities were dominated by Acidobacteria,Proteobacteria,Chloroflexi,Firmicutes,Verrucomicrobia and Actinobacteria.NMDS and cluster analysis showed that different nitrogen application treatments did not result in obvious separation of bacterial community structure.LDA analysis showed that treatmentⅢhad the highest number of differentiated species.Chloroflexi could be used as indicator species after nitrogen application.100 g·m^(-2)·a^(-1) nitrogen application was the most beneficial to bacterial growth.150 g·m^(-2)·a^(-1) nitrogen application had certain inhibitory effect on soil bacterial growth.

关 键 词：氮沉降 土壤细菌 多样性 群落结构 猫儿山 

分 类 号：S718.55[农业科学—林学] S714.8