棱果花组培快繁体系建立  

Tissue Culture and Rapid Propagation System of Barthea barthei

作　　者：廖焕琴 杨晓慧[1,2] 徐放 杨会肖[1,2] 陈新宇 徐斌[1,2] Liao Huanqin;Yang Xiaohui;Xu Fang;Yang Huixiao;Chen Xinyu;Xu Bin(Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China;Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou,Guangdong 510520,China)

机构地区：[1]广东省林业科学研究院,广东广州510520 [2]广东省森林培育与保护利用重点实验室,广东广州510520

出　　处：《广西林业科学》2024年第4期501-506,共6页Guangxi Forestry Science

基　　金：广东省林业科技创新项目(2020KJCX002)。

摘　　要：为建立棱果花(Barthea barthei)组培快繁体系,推动棱果花优良种质资源快速繁育进程,以棱果花优树茎段作为外植体,研究茎段木质化程度、叶片保留方式和灭菌时间及培养基对棱果花组培快繁的影响。结果表明,采用1/2MS+0.5 mg/L6-BA作为培养基,选择嫩枝作为外植体,棱果花茎段存活率最高(75.69%)。采用保留全叶方式,经75%酒精处理10 s,0.1%升汞灭菌5 min,棱果花茎段存活率最高(91.67%)。棱果花初代培养的最佳培养基为1/2MS+0.5 mg/L 6-BA,萌芽率最高(83.33%)。棱果花增殖培养的最佳培养基为WPM+0.5 mg/L 6-BA,增殖系数最高(3.00)。棱果花生根培养的最佳培养基为1/2MS+1.0 mg/L IBA,生根率最高(95.24%)。棱果花移栽基质配方为椰糠∶泥炭土∶珍珠岩=3∶3∶1(V∶V∶V)时,成活率达85%。In order to establish tissue culture and fast propagation system for Barthea barthei and promote rapid propagation of excellent germplasm resources of B.barthei,taking stem segments of B.barthei as explants,effects of lignification degree of stem segments,remained leaf methods and disinfection time as well as culture mediums on tissue culture and fast propagation of B.barthei were studied.Results showed that stem segments of B.barthei had the highest survival rate(75.69%)by using 1/2MS+0.5 mg/L 6-BA as culture medium and selecting shoots as explants.Stem segments of B.barthei had the highest survival rate(91.67%)by retaining whole leaves,treated with 75%alcohol for 10 s and sterilized with 0.1%ascending mercury for 5 min.Optimum culture medium for primary culture of B.barthei was 1/2MS+0.5 mg/L 6-BA with the highest germination rate(83.33%).Optimum culture medium for proliferation culture of B.barthei was WPM+0.5 mg/L 6-BA with the highest proliferation coefficient(3.00).Optimum culture medium for rooting culture of B.barthei was 1/2 MS+1.0 mg/L IBA with the highest rooting rate(95.24%).Substrate formulation for B.barthei transplanting was coco coir∶peat soil∶perlite=3∶3∶1(V∶V∶V)with 85%survival rate.

关 键 词：观赏植物 组织培养 野牡丹科 棱果花 

分 类 号：S722.8[农业科学—林木遗传育种]