Value of Metagenomic Next-Generation Sequencing in Clinical Diagnosis and Targeted Therapy of Nontuberculous Mycobacteria Infection  

作　　者：Lanlan Xiao Shuai Zhao Hongjuan Wang Liang Yu Jing Guo Xinyi Wu Xiaowei Xu 

机构地区：[1]State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,National Clinical Research Center for Infectious Diseases,National Medical Center for Infectious Diseases,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,China [2]Affiliated Hospital of Nantong University,Nantong,China [3]Zhejiang University City College,Hangzhou,China

出　　处：《Infectious Microbes & Diseases》2024年第2期100-102,共3页感染微生物与疾病（英文）

基　　金：the Science&Technology Key Program of Zhejiang China(2017C03051);Key Research and Development Program of Zhejiang Province(2020c03123-1).

摘　　要：The purpose of this study was to assess the value of metagenomic next-generation sequencing(mNGS)for rapid diagnosis of diseases caused by nontuberculous mycobacteria(NTM).We retrospectively reviewed four NTM-infected cases diagnosed by smear microscopy,mycobacterial culture and mNGS methods.We found that the mNGS method not only had a shorter detection turnaround time(3–4 days)than Mycobacterium culture(15–20 days)but also had higher sensitivity and specificity to identify NTM compared with conventional detection methods.In addition,mNGS was able to identify coinfections by NTM and other bacteria,fungi or viruses.Overall,diagnosis of NTM by mNGS can provide timely and precise guidance for subsequent clinical treatment of NTM infections.

关 键 词：nontuberculous mycobacteria metagenomic next-generation sequencing mycobacterial culture turnaround time 

分 类 号：R446.5[医药卫生—诊断学]