观赏桃黄酮合成酶CHS基因家族分析  

Analysis of the CHS Gene Family in Ornamental Peach

作　　者：帅泽宇 徐曼[1] 屈成 张文斗 Shuai Zeyu;Xu Man;Qu Cheng;Zhang Wendou(Peach Blossom Engineering Technology Research Center,Changde Vocational and Technical College,Changde 415000,Hunan,China;Hunan Biological And Electromechanical Polytechnic,Changsha 410127,Hunan,China)

机构地区：[1]常德职业技术学院桃花工程技术研究中心,湖南常德415000 [2]湖南生物机电职业技术学院,湖南长沙410127

出　　处：《绿色科技》2024年第14期259-263,共5页Journal of Green Science and Technology

基　　金：常德职业技术学院青年项目(编号:ZY2202)。

摘　　要：查尔酮合酶(chalcone synthase,CHS)是植物类黄酮生物合成路径中的关键酶,对观赏植物花色的形成具有决定性作用。研究旨在全面分析观赏桃PpCHSs基因家族,探究其在观赏植物花色形成中的分子机制,为育种提供理论支持。通过Uniprot和GDR数据库获取并比对CHS蛋白序列,使用TBtools进行基因鉴定和染色体共线性分析,MEGA 7.0构建系统发育树,NCBI CDD分析蛋白保守结构域。确认观赏桃中17个CHS同源基因,主要分布在G1染色体。共线性分析显示观赏桃CHS基因与拟南芥存在一对多关系,反映基因家族扩张。系统发生树将基因分为三类,与不同功能特化相关。蛋白结构域分析表明功能上的保守性,揭示了CHS基因家族在观赏桃花色形成中的关键作用,为基因编辑和育种提供理论基础,促进观赏植物产业发展。Chalcone synthase(CHS)is a key enzyme in the plant flavonoid biosynthetic pathway,playing a crucial role in the formation of floral colors in ornamental plants.This study aims to comprehensively analyze the PpCHSs gene family in ornamental peach and explore its molecular mechanisms in the formation of floral colors,providing theoretical support for breeding.CHS protein sequences were obtained and compared using the Uniprot and GDR databases.Gene identification and chromosomal collinearity analysis were performed using TBtools.Phylogenetic trees were constructed with MEGA 7.0,and protein conserved domains were analyzed using NCBI CDD.This confirmed 17 CHS homologous genes in ornamental peach,mainly distributed on chromosome G1.Collinearity analysis showed a one-to-many relationship between the ornamental peach CHS genes and Arabidopsis,reflecting gene family expansion.The phylogenetic tree categorized the genes into three groups,related to different functional specializations.Analysis of protein domains indicated conservation of function.This reveals the key role of the CHS gene family in the formation of floral colors in ornamental peach,providing a theoretical basis for gene editing and breeding,and promoting the development of the ornamental plant industry.

关 键 词：查尔酮合酶 类黄酮生物合成 基因家族 观赏桃 分子育种 

分 类 号：S722[农业科学—林木遗传育种]