玉屏风散加味方化学成分鉴定  

Identification of chemical constituents of modified Yupingfengsan formula

作　　者：刘翠珍 冯思琪 林秀娟 卢琴 黄育生[2,3] 张丽娟 李得堂 LIU Cuizhen;FENG Siqi;LIN Xiujuan;LU Qin;HUANG Yusheng;ZHANG Lijuan;LI Detang(School of First Clinical Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Lingnan Medical Research Center,Guangzhou University of Chinese Medicine,Guangzhou 510407,China;Guangdong Clinical Research Academy of Chinese Medicine,Guangzhou 510405,China;Dept.of Otorhinolaryngology,the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Dept.of Pharmacy,the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一临床医学院,广州510405 [2]广州中医药大学岭南医学研究中心,广州510407 [3]广东省中医临床研究院,广州510405 [4]广州中医药大学第一附属医院耳鼻喉科,广州510405 [5]广州中医药大学第一附属医院药学部,广州510405

出　　处：《中国药房》2024年第18期2225-2231,共7页China Pharmacy

基　　金：国家自然科学基金面上项目(No.82374526);广州市科技计划项目(No.2023A03J0299,No.202201020457);广州中医药大学第一附属医院2023年中青年骨干人才[菁英人才(临床型)]培育项目。

摘　　要：目的鉴定玉屏风散加味方的化学成分。方法采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术。以Waters BEH C18为色谱柱,乙腈(A)-0.1%甲酸溶液(B)为流动相进行梯度洗脱;采用加热电喷雾离子源进行正、负离子模式扫描,扫描范围为m/z 50~1500,喷雾电压为2 kV(正离子模式)、1.5 kV(负离子模式)。通过查阅文献收集玉屏风散加味方的化学成分信息,建立数据库;结合上述成分数据库、相关文献以及对照品的色谱、质谱信息对该方成分结构进行鉴定。结果与结论从玉屏风散加味方中共鉴定出114个化学成分,包括黄酮类(31个)、苯丙素类(39个)、皂苷类(5个)、萜类(8个)、色原酮类(3个)、姜辣素类(3个)等;通过与对照品比对,最终确定了8个成分,分别为木兰花碱、毛蕊异黄酮、毛蕊异黄酮葡萄糖苷、升麻素、5-O-甲基维斯阿米醇苷、亥茅酚苷、木犀草素、芒柄花素;主要涉及糖基断裂、逆狄尔斯-阿尔德重排、糖基丢失、中性分子丢失等裂解途径。OBJECTIVE To identify the chemical constituents of the modified Yupingfengsan formula.METHODS UPLC-QExactive Orbitrap-MS technology was adopted.The separation was performed on Waters BEH C18 column with acetonitrile(A)-0.1%formic acid solution(B)as mobile phase for gradient elution.The heating electrospray ionization was used for positive and negative ion mode scanning.The scanning range was m/z 50-1500,and the spray voltage was 2 kV(positive ion mode)and 1.5 k V(negative ion mode).The information of chemical constituents of modified Yupingfengsan formula was collected through literature review to establish a database;the structure of the constituent was identified based on the above database,relevant literature,and chromatography and mass spectrometry information of reference standards.RESULTS&CONCLUSIONS Totally 114 chemical constituents were identified from modified Yupingfengsan formula,including 31 flavonoids,39 phenylpropanoids,5saponins,8 terpenoids,3 chromones,3 curcuminoids,etc.Based on the comparison of reference standards,8 constituents were ultimately determined,including magnoflorine,calycosin,calycosin glycoside,cimifugin,5-O-methylvisammioside,sec-Oglucosylhamaudol,luteolin and mangiferin.These constituents mainly involved glycosylation cleavage,retro Diels-Alder fragmentation,glycosylation loss,neutral molecule loss and other fragmentation pathways.

关 键 词：玉屏风散加味方 化学成分 黄酮类 苯丙素类 裂解规律 

分 类 号：R917[医药卫生—药物分析学]