一株牙鲆源哈维氏弧菌的分离鉴定及抗性研究  

Isolation,identification and resistance of Vibrio harveyi isolated from Japanese flounder(Paralichthys olivaceus)

作　　者：高培宇 郭铠莹 殷蕊 宫春光[2] GAO Peiyu;GUO Kaiying;YIN Rui;GONG Chunguang(Zhangjiakou Aquatic Technology Extension Station,Zhangjiakou 075000,China;Ocean College of Hebei Agricultural University,Qinhuangdao 066003,China;Hebei Academy of Marine and Aquatic Sciences,Qinhuangdao 066201,China)

机构地区：[1]张家口市水产技术推广站,河北张家口075000 [2]河北农业大学海洋学院,河北秦皇岛066003 [3]河北省海洋与水产科学研究院,河北秦皇岛066200

出　　处：《河北渔业》2024年第9期1-5,共5页

基　　金：河北省现代农业产业技术体系特色海产品创新团队疫病防控及质量控制岗位(项目编号:HBCT2023220207);河北省现代农业产业技术体系淡水养殖创新团队张家口综合试验推广站(项目编号:HBCT2024300404)。

摘　　要：自秦皇岛昌黎养殖的患病牙鲆(Paralichthys olivaceus)体内分离获得一株革兰氏阴性菌,对其形态特征、生理生化特性及16S rDNA序列进行分析,鉴定其为哈维氏弧菌(Vibrio harveyi);对分离菌株进行了30种抗菌药的药敏试验和11株益生菌的拮抗试验,得出此分离菌株对米诺环素、左氧氟沙星、头孢他啶、氟苯尼考、头孢曲松、诺氟沙星、环丙沙星、强力霉素、复方新诺明等13种药物高度敏感,对克林霉素、青霉素、氨苄西林、林可霉素、苯唑西林等9种药物表现为不同程度的耐药性;地衣芽孢杆菌和枯草芽孢杆菌2种益生菌在一定浓度下对其具有拮抗作用。A Gram-negative bacterium was isolated from diseased cultured Japanese flounder(Paralichthys olivaceus)in Changli,Qinhuangdao.Its morphological characteristics,physiological and biochemical properties,as well as its 16S rDNA sequence were analyzed,identifying it as Vibrio harveyi.The isolated strain underwent antibiotic susceptibility testing with 30 antibiotics and an antagonistic experiment with 11 probiotics.The results revealed that the isolated strain exhibited high sensitivity to 13 antibiotics,including minocycline,levofloxacin,cefotaxime,florfenicol,norfloxacin,ciprofloxacin,doxycycline and compound sulfadiazine among others;while showing varying degrees of resistance to 9 antibiotics such as clindamycin,penicillin and ampicillin.Two probiotics,Bacillus licheniformis and B.subtilis,demonstrated antagonistic effects on the strain at certain concentrations.

关 键 词：牙鲆(Paralichthys olivaceus) 哈维氏弧菌(Vibrio harveyi) 分离鉴定 药敏试验 拮抗试验 

分 类 号：S943[农业科学—水产养殖]