过表达野生大豆耐盐碱基因GsGSTU13提高了水稻苗期耐盐碱性  

Overexpression of wild soybean salt-alkali tolerance gene GsGSTU13 increases salt-alkaline tolerance in rice seedlings

作　　者：李宛鸿 胡冰霜 孙晓丽 才晓溪 孙明哲 LI Wan-Hong;HU Bing-Shuang;SUN Xiao-Li;CAI Xiao-Xi;SUN Ming-Zhe(College of Agriculture,Heilongjiang Bayi Agriculture University/Crop Stress Molecular Biology Laboratory,Daqing 163316,Heilongjiang,China)

机构地区：[1]黑龙江八一农垦大学农学院/作物逆境分子生物学实验室,黑龙江大庆163316

出　　处：《作物学报》2024年第10期2458-2467,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(U20A2025,31971826);黑龙江省自然科学基金项目(YQ2023C035)资助。

摘　　要：谷胱甘肽S-转移酶(glutathione S-transferases,GSTs)是一类高度保守的功能酶,在植物对非生物胁迫应答过程中发挥重要作用。本研究通过生物信息学分析发现,野生大豆GsGSTU13与水稻GSTU17蛋白序列相似性最高,均含有高度保守的结构域。为探究GsGSTU13基因对水稻耐盐碱性的影响,通过遗传转化、PCR、RT-PCR和GST活性检测,获得了2个GsGSTU13过表达转基因水稻纯合株系。苗期耐盐碱性分析显示,200 mmol L^(-1) NaHCO_(3)处理下,GsGSTU13转基因株系的活性氧积累显著低于野生型,而存活率、相对含水量、超氧化物歧化酶、过氧化物酶、过氧化氢酶和GST活性均显著高于野生型。综上,在水稻中过表达GsGSTU13基因增强了活性氧清除能力,提高了转基因水稻耐盐碱性,为耐盐碱水稻新种质创制提供了参考。Glutathione S-transferases(GSTs)are a class of highly conserved enzymes that play crucial roles in plant responses to environmental stresses.Bioinformatic analysis has revealed that Glycine soja GsGSTU13,which positively regulates salt-alkaline tolerance,shares the highest sequence identity with the OsGSTU17 protein.To investigate the potential contribution of GsGSTU13 to rice salt-alkaline tolerance,we transformed GsGSTU13 into rice and obtained two homozygous transgenic lines with significantly elevated GST activity.Phenotypic assays showed that after treatment with 200 mmol L^(-1) NaHCO_(3),the accumulation of reactive oxygen species was significantly lower in GsGSTU13 transgenic lines compared to wild-type.Additionally,the survival rates,relative water contents,and the activities of superoxide dismutases,peroxidases,catalases,and GSTs were significantly higher in GsGSTU13 transgenic lines than in the wild-type.In summary,overexpression of GsGSTU13 in rice enhanced salt-alkaline tolerance by promoting ROS scavenging,which could facilitate the breeding of new rice cultivars with improved tolerance to salt-alkaline stress.

关 键 词：水稻 野生大豆 耐盐碱性 谷胱甘肽S-转移酶 GsGSTU13 

分 类 号：S511[农业科学—作物学]