深水稻全生育期耐盐性状的QTL定位  

QTL mapping for salt tolerance traits throughout the entire growth period of deep-water rice

作　　者：夏秀忠 张宗琼 农保选 冯锐 郭辉 陈灿 梁树辉 荘洁 廖祖宇 宋国显 杨行海 李丹婷 XIA Xiu-Zhong;ZHANG Zong-Qiong;NONG Bao-Xuan;FENG Rui;GUO Hui;CHEN Can;LIANG Shu-Hui;ZHUANG Jie;LIAO Zu-Yu;SONG Guo-Xian;YANG Xing-Hai;LI Dan-Ting(Rice Research Institute,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding,Nanning 530007,Guangxi,China;Qinzhou Institute of Agricultural Sciences,Qinzhou 535000,Guangxi,China)

机构地区：[1]广西壮族自治区农业科学院水稻研究所/广西水稻遗传育种重点实验室,广西南宁530007 [2]钦州市农业科学研究所,广西钦州535000

出　　处：《作物学报》2024年第10期2493-2502,共10页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2021YFD1200505-2);国家自然科学基金项目(32260487,32360519);广西科技重大专项(桂科AA23073004);广西科技计划项目(桂科AB21238009);广西农业科学院基本科研业务专项(桂农科2021YT030)资助。

摘　　要：盐胁迫是许多沿海地区水稻生产的主要制约因素,尤其是沿海地区的咸淡水交汇区域。耐盐性是一种复杂的性状,可以通过QTL定位来帮助耐盐育种,以培育更高耐盐性的水稻品种。本研究供体亲本为沿海深水稻品种赤禾,受体亲本为美国水稻品种Lemont,杂交获得174份F9代的重组自交系,在芽期、苗期和生殖生长期分别利用浓度为15 g L^(-1)、5 g L^(-1)和5~6 g L^(-1)的NaCl进行胁迫,通过芽期相对发芽率、苗期耐盐性评级和生殖生长期的7个表型性状为基础数据,利用142个SSR分子标记绘制连锁遗传图并进行QTL分析。鉴定结果发现,赤禾在芽期表现敏盐,在苗期和生殖生长期表现耐盐;Lemont相反。3个生长时期分别有70.11%、50.57%和60.34%的品系表现为弱耐盐性,而且耐盐性为弱的负相关。本研究共鉴定出33个LOD值为2.52~10.32的QTL,解释0.06%~13.68%的表型遗传变异,解释最大遗传变异的QTL均由耐盐亲本贡献,其中芽期4个、苗期6个和生殖生长期23个位点,并在生殖生长期发现4个QTL重叠区域。这些QTL可以进一步研究,不仅为提高水稻育种的耐盐性提供了新的遗传资源,还有助于在水稻耐盐育种中,提高水稻品种的耐盐性。Salinity stress is a major constraint to rice production in many coastal regions,particularly in the salt-fresh water interface areas.Salt tolerance in rice is a complex trait that can be localized through quantitative trait loci(QTL)mapping,which can facilitate the breeding of rice varieties with enhanced salt tolerance.In this study,an F9 mapping population consisting of 174 recombinant inbred lines was developed from a cross between the coastal deep-water rice variety Chihe(donor parent)and the U.S.rice variety Lemont(receptor parent).The population was subjected to salinity stress during the germination,seedling,and reproductive stages with NaCl concentrations of 15 g L^(-1),5 g L^(-1),and 5-6 g L^(-1),respectively.Data were collected on relative germination rates,seedling salt tolerance grades,and seven phenotypic traits during the reproductive stages.Linkage genetic mapping and QTL mapping were performed using 142 simple sequence repeat(SSR)markers.The results indicated that Chihe was salt-sensitive at the germination stage but exhibited salt tolerance at the seedling and reproductive stages,while Lemont lines were consistently salt-sensitive.Approximately 70.11%,50.57%,and 60.34%of the lines showed salt sensitivity in the germination,seedling,and reproductive stages,respectively,with a weak negative correlation of salt tolerance across the stages.A total of 33 QTLs were identified,with LOD values ranging from 2.52 to 10.32 and phenotypic variation explained ranging from 0.06%to 13.68%.Specifically,4 QTLs were identified for the germination stage,6 for the seedling stage,and 23 for the reproductive stage.Four overlapping QTLs were identified at the reproductive stage,and the QTLs contributing to the largest phenotypic variation were all derived from the salt-tolerant parent.Further investigation of these QTLs can provide new genetic resources for improving salt tolerance in rice breeding,thereby aiding the development of rice varieties with enhanced tolerance to salinity stress.

关 键 词：全生育期 耐盐 深水稻 鉴定评价 数量性状位点 

分 类 号：S511[农业科学—作物学]