大麦HvMBF1c耐盐机制分析  

Salt tolerance analysis of HvMBF1c in barley

作　　者：湛潇潇 冯举伶 张震欢 张宏 汪军成[1,2] 李葆春[1,3] 杨轲[1,2] 司二静[1,2] 孟亚雄[1,2] 马小乐[1,2] 王化俊[1,2] 姚立蓉 ZHAN Xiao-Xiao;FENG Ju-Ling;ZHANG Zhen-Huan;ZHANG Hong;WANG Jun-Cheng;LIBao-Chun;YANG Ke;SI Er-Jing;MENG Ya-Xiong;MA Xiao-Le;WANG Hua-Jun;YAOLi-Rong(State Key Laboratory of Crop Science in Arid Habitat Jointly Established by the Ministry of Province and Ministry of Finance/Key Laboratory of Crop Genetic Improvement and Germplasm Innovation in Gansu Province,Lanzhou 730070,Gansu,China;Agronomy College,Gansu Agricultural University,Lanzhou 730070,Gansu,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《作物学报》2024年第10期2503-2514,共12页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(大麦青稞,CARS-05-03B-03);国家自然科学基金项目(30771331,32160496);甘肃农业大学学生科研训练计划(202301047,202210733008,S202210733003);甘肃省青年科技基金计划(20JR5RA010);甘肃省教育厅创新基金(2021A-055);甘肃省教育厅产业支撑计划项目(2021CYZC-12)资助。

摘　　要：大麦HvMBF1c基因参与调控植物盐胁迫表达,为探究其对盐胁迫的响应模式,本文对大麦HvMBF1基因进行生物信息学分析,并以中川大麦(耐盐型)、GN18(盐敏感型)及WT与转HvMBF1c基因拟南芥为材料,分别取200 mmol L^(-1) NaCl胁迫0 h、6 h、12 h、24 h、48 h及72 h样品,对其进行qRT-PCR分析和生理指标的测定。结果显示,大麦HvMBF1c基因与小麦TaMBF1c亲缘关系最近,位于7H染色体上。HvMBF1c的外显子比HvMBF1a和HvMBF1b的大,且HvMBF1a和HvMBF1b两者具有典型的motif 4结构域,而HvMBF1c基因具有独特的motif 5结构域。HvMBF1c基因的启动子序列含有光响应、根特定调节元件和缺氧特异性诱导增强元件,与小麦2号、3号和7号染色体存在同源性。qRT-PCR分析表明,随着盐处理时间的延长,大麦HvMBF1c基因在2份种质材料苗期、拔节期、抽穗期和灌浆期的表达量呈上升趋势,且中川大麦中该基因的表达量高于GN18中的表达量;转HvMBF1c拟南芥中其表达量随着盐处理时间的延长而升高。盐胁迫下,中川大麦苗期、拔节期、抽穗期及灌浆期的SOD、POD、CAT、MDA和可溶性蛋白低于GN18,转基因拟南芥中各生理指标的变化量低于WT。本研究结果为进一步探索HvMBF1c的功能提供参考。The HvMBF1c gene in barley is implicated in the plant’s response to salt stress.To investigate the HvMBF1c response mechanism to salt stress,we conducted bioinformatics analysis of the HvMBF1 gene,qRT-PCR analysis,and physiological index determination in Nakagawa barley(salt-tolerant type),GN18(salt-sensitive type),wild-type(WT)and transgenic HvMBF1c Arabidopsis thaliana treated with 200 mmol L^(-1) NaCl for 0,6,12,24,48,and 72 hours.The results showed that the barley HvMBF1c gene is closely related to the wheat TaMBF1c gene,and is located on chromosome 7H.The exon of HvMBF1c is larger than those of HvMBF1a and HvMBF1b,with HvMBF1a and HvMBF1b containing a typical motif 4 domain,while the HvMBF1c gene has a unique motif 5 domain.The promoter sequence of the HvMBF1c gene includes photoreactive,root-specific regulatory elements,and hypoxia-specific inducible enhancing elements,homologous to wheat chromosomes 2,3,and 7.qRT-PCR analysis revealed significant upregulation of HvMBF1c expression in two barley genotypes at the seedling,jointing,heading,and filling stages,with higher expression in Nakagawa barley compared to GN18 as salt treatment duration increased.Similarly,the expression of HvMBF1c in Arabidopsis thaliana also increased with prolonged salt treatment time.Physiological indices of Nakagawa barley at each growth stage were lower than those in GN18,and transgenic Arabidopsis exhibited lower indices compared to WT under different salt stress conditions.These findings provide a foundation for further exploration of the HvMBF1c gene function.

关 键 词：大麦 HvMBF1c 生理指标 共线性分析 表达量 

分 类 号：S512.3[农业科学—作物学]