基于BDNF/TrkB通路探讨电针对脑缺血再灌注损伤大鼠学习记忆功能及突触可塑性的影响  

Effects of electroacupuncture on learning-memory function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury based on the BDNF/TrkB pathway

作　　者：程静 杨一涵 陈进城 饶婷[2,3] 江一静 杨珊莉 CHENG Jing;YANG Yihan;CHEN Jincheng;RAO Ting;JIANG Yijing;YANG Shanli(First Clinical Medical College of Fujian University of TCM,Fuzhou 350122,China;College of Rehabilitation Medicine,Fujian University of TCM;Affiliated Rehabilitation Hospital of Fujian University of TCM,Fuzhou 350003;Fujian Provincial Key Laboratory of Rehabilitation Technology,Fuzhou 350003)

机构地区：[1]福建中医药大学第一临床医学院,福州350122 [2]福建中医药大学康复医学院 [3]福建中医药大学附属康复医院,福州350003 [4]福建省康复技术重点实验室,福州350003

出　　处：《中国针灸》2024年第9期1037-1045,共9页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:82074513;福建省自然科学基金计划项目:2021J01955;福建省中医康复学科开放课题重点项目:XKF2023011。

摘　　要：目的:观察电针“百会”“四神聪”对脑缺血再灌注损伤(CIRI)大鼠缺血侧海马脑源性神经营养因子(BDNF)/酪氨酸激酶受体B(TrkB)通路、突触素(SYN)表达及白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)含量的影响,探究电针改善CIRI后学习记忆功能的作用及机制。方法:将48只SPF级雄性SD大鼠随机分为假手术组、模型组、电针组和非穴组,每组12只。模型组、电针组和非穴组大鼠采用改良Zea Longa线栓法制备CIRI模型。电针组予电针“四神聪”“百会”,疏密波,频率2 Hz/10 Hz,电流强度1 mA;非穴组电针双侧肋下非经非穴点,电针参数同电针组。两组干预均每次30 min,每天1次,持续7 d。干预期间,于每日固定时间测量大鼠体质量;于干预第1、3、7天观察各组大鼠神经功能缺损情况。干预前后采用小动物磁共振成像技术测量大鼠脑梗死体积。干预后,采用Morris水迷宫评价大鼠学习记忆功能;HE染色观察大鼠海马形态;免疫组化法检测大鼠缺血侧海马SYN阳性表达;Western blot法检测大鼠缺血侧海马BDNF、TrkB、SYN蛋白表达;ELISA法检测大鼠缺血侧海马IL-1β、IL-18含量。结果:干预第2~7天,与假手术组比较,模型组大鼠体质量下降(P<0.01);与模型组和非穴组比较,电针组大鼠体质量增加(P<0.01)。干预第1天,与假手术组比较,模型组、电针组和非穴组大鼠神经功能评分升高(P<0.01);干预第3、7天,模型组大鼠神经功能评分高于假手术组(P<0.01);干预第7天,电针组大鼠神经功能评分低于模型组与非穴组(P<0.05)。与假手术组比较,模型组大鼠逃避潜伏期延长(P<0.05),穿越平台次数减少(P<0.01);与模型组和非穴组比较,电针组大鼠逃避潜伏期缩短(P<0.05),穿越平台次数增加(P<0.01)。干预前,模型组、电针组和非穴组大鼠左侧脑室出现明显高信号梗死灶;干预后与模型组和非穴组比较,电针组大鼠脑梗死体积占比下降(P<0.01)。�Objective To observe the effects of electroacupuncture(EA)at"Baihui"(GV 20)and"Sishencong"(EX-HN 1)on the expression of brain-derived neurotrophic factor(BDNF)/tyrosine kinase receptor B(TrkB)pathway,synaptophysin(SYN),and the levels of interleukin-1β(IL-1β)and interleukin-18(IL-18)in the hippocampus of the ischemic side in rats with cerebral ischemia-reperfusion injury(CIRI),and to explore the effects and action mechanism of EA on post-CIRI learning-memory function.Methods Forty-eight SPF-grade male SD rats were randomly divided into a sham operation group,a model group,an EA group,and a non-acupoint group,with 12 rats in each group.The CIRI model was established in the model group,the EA group,and the non-acupoint group using the modified ZeaLonga suture method.The rats in the EA group were treated with EA at"Sishencong"(EX-HN 1)and"Baihui"(GV 20),with disperse-dense wave at frequency of 2 Hz/10 Hz and intensity of 1 mA.The rats in the non-acupoint group were treated with EA at non-meridian and non-acupoint points under the ribs bilaterally with the same parameters as the EA group.EA were conducted for 30 min each session,once daily,for 7 days.During the intervention,body weight was measured daily at a fixed time,and neurological deficits were assessed on the 1st,3rd,and 7th days into intervention.Brain infarct volume was measured using small animal magnetic resonance imaging before and after the intervention.After the intervention,learning-memory function were evaluated using the Morris water maze.Hippocampal morphology was observed with HE staining.The positive expression of SYN in the hippocampus of the ischemic side was detected by immunohistochemistry.BDNF,TrkB,and SYN protein expressions in the hippocampus of the ischemic side were detected by Western blot.IL-1βand IL-18 levels in the hippocampus of the ischemic side were measured by ELISA.Results From the 2nd to the 7th day into intervention,compared with the sham operation group,the body weight of rats in the model group was decreased(P<0.01);compare

关 键 词：脑缺血再灌注损伤 卒中后认知功能障碍 电针 学习记忆功能 突触可塑性 脑源性神经营养因子(BDNF)/酪氨酸激酶受体B(TrkB)通路 

分 类 号：R245[医药卫生—针灸推拿学]